Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 49062..49798 Replicon plasmid pCAV1015-114
Accession NZ_CP017930
Organism Klebsiella oxytoca strain CAV1015

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AGF18_RS29640 Protein ID WP_003026803.1
Coordinates 49062..49544 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AGF18_RS29645 Protein ID WP_003026799.1
Coordinates 49532..49798 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGF18_RS29615 44375..45190 - 816 WP_012695470.1 aminoglycoside O-phosphotransferase APH(3')-Ia -
AGF18_RS29620 45351..46319 - 969 WP_013815099.1 IS5-like element IS903B family transposase -
AGF18_RS29625 46351..46722 - 372 Protein_51 IS5/IS1182 family transposase -
AGF18_RS29630 46797..47429 + 633 WP_001567369.1 hypothetical protein -
AGF18_RS29635 47458..48861 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AGF18_RS29640 49062..49544 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AGF18_RS29645 49532..49798 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AGF18_RS29650 50243..50473 - 231 WP_004206660.1 hypothetical protein -
AGF18_RS29655 50487..50690 - 204 WP_004213596.1 hemolysin expression modulator Hha -
AGF18_RS29660 50751..51242 - 492 WP_004206662.1 hypothetical protein -
AGF18_RS29665 51664..52632 + 969 WP_176678774.1 IS5 family transposase -
AGF18_RS32455 52684..52875 - 192 Protein_60 IS1 family transposase -
AGF18_RS29670 53106..53336 - 231 Protein_61 oxidoreductase -
AGF18_RS29675 53363..54331 + 969 WP_077257929.1 IS5-like element IS903B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qnrB2 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia / aadA2 pla 1..114139 114139
- inside IScluster/Tn aph(3')-Ia - 38719..54331 15612


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T68366 WP_003026803.1 NZ_CP017930:c49544-49062 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T68366 NZ_CP017930:c49544-49062 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT68366 WP_003026799.1 NZ_CP017930:c49798-49532 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT68366 NZ_CP017930:c49798-49532 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References