Detailed information of TA system TA068366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 49062..49798
• Replicon: plasmid pCAV1015-114
• Accession: NZ_CP017930
• Organism: Klebsiella oxytoca strain CAV1015

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AGF18_RS29640
• Protein ID: WP_003026803.1
• Coordinates: 49062..49544 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AGF18_RS29645
• Protein ID: WP_003026799.1
• Coordinates: 49532..49798 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGF18_RS29615	44375..45190	-	816	WP_012695470.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
AGF18_RS29620	45351..46319	-	969	WP_013815099.1	IS5-like element IS903B family transposase	-
AGF18_RS29625	46351..46722	-	372	Protein_51	IS5/IS1182 family transposase	-
AGF18_RS29630	46797..47429	+	633	WP_001567369.1	hypothetical protein	-
AGF18_RS29635	47458..48861	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
AGF18_RS29640	49062..49544	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AGF18_RS29645	49532..49798	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AGF18_RS29650	50243..50473	-	231	WP_004206660.1	hypothetical protein	-
AGF18_RS29655	50487..50690	-	204	WP_004213596.1	hemolysin expression modulator Hha	-
AGF18_RS29660	50751..51242	-	492	WP_004206662.1	hypothetical protein	-
AGF18_RS29665	51664..52632	+	969	WP_176678774.1	IS5 family transposase	-
AGF18_RS32455	52684..52875	-	192	Protein_60	IS1 family transposase	-
AGF18_RS29670	53106..53336	-	231	Protein_61	oxidoreductase	-
AGF18_RS29675	53363..54331	+	969	WP_077257929.1	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qnrB2 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia / aadA2	pla	1..114139	114139
	-	inside	IScluster/Tn	aph(3')-Ia	-	38719..54331	15612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T68366 WP_003026803.1 NZ_CP017930:c49544-49062 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T68366 NZ_CP017930:c49544-49062 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT68366 WP_003026799.1 NZ_CP017930:c49798-49532 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT68366 NZ_CP017930:c49798-49532 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66