Detailed information of TA system TA068365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 5975688..5976307
• Replicon: chromosome
• Accession: NZ_CP017928
• Organism: Klebsiella oxytoca strain CAV1015

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: AGF18_RS27910
• Protein ID: WP_004099646.1
• Coordinates: 5975688..5975906 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AGF18_RS27915
• Protein ID: WP_004099648.1
• Coordinates: 5975933..5976307 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGF18_RS27885	5971724..5971987	+	264	WP_004099638.1	type B 50S ribosomal protein L31	-
AGF18_RS32270	5971987..5972127	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
AGF18_RS27890	5972124..5972822	-	699	WP_004099639.1	GNAT family N-acetyltransferase	-
AGF18_RS27895	5972925..5974379	+	1455	WP_024273853.1	PLP-dependent aminotransferase family protein	-
AGF18_RS27900	5974354..5974824	-	471	WP_004111038.1	YlaC family protein	-
AGF18_RS27905	5974961..5975527	-	567	WP_004099644.1	maltose O-acetyltransferase	-
AGF18_RS27910	5975688..5975906	-	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
AGF18_RS27915	5975933..5976307	-	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
AGF18_RS27920	5976789..5979935	-	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
AGF18_RS27925	5979958..5981151	-	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T68365 WP_004099646.1 NZ_CP017928:c5975906-5975688 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T68365 NZ_CP017928:c5975906-5975688 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT68365 WP_004099648.1 NZ_CP017928:c5976307-5975933 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT68365 NZ_CP017928:c5976307-5975933 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H713