Detailed information of TA system TA068362

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3104701..3105473
• Replicon: chromosome
• Accession: NZ_CP017928
• Organism: Klebsiella oxytoca strain CAV1015

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AGF18_RS14375
• Protein ID: WP_165569689.1
• Coordinates: 3105105..3105473 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AGF18_RS14370
• Protein ID: WP_047720742.1
• Coordinates: 3104701..3105060 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGF18_RS14330	3099929..3100786	+	858	WP_025107340.1	hypothetical protein	-
AGF18_RS14335	3101064..3101528	+	465	WP_004136903.1	hypothetical protein	-
AGF18_RS14340	3101583..3101942	+	360	WP_004136901.1	hypothetical protein	-
AGF18_RS14345	3102028..3102240	+	213	WP_047720745.1	hypothetical protein	-
AGF18_RS14350	3102347..3103168	+	822	WP_004136895.1	DUF945 domain-containing protein	-
AGF18_RS14355	3103247..3103720	+	474	WP_047720744.1	antirestriction protein	-
AGF18_RS14360	3103961..3104443	+	483	WP_047720743.1	DNA repair protein RadC	-
AGF18_RS14365	3104457..3104678	+	222	WP_032938028.1	DUF987 domain-containing protein	-
AGF18_RS14370	3104701..3105060	+	360	WP_047720742.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AGF18_RS14375	3105105..3105473	+	369	WP_165569689.1	TA system toxin CbtA family protein	Toxin
AGF18_RS14380	3105553..3105873	-	321	WP_047058025.1	hypothetical protein	-
AGF18_RS14385	3106012..3107001	-	990	WP_047720741.1	glycosyltransferase	-
AGF18_RS14390	3107765..3108085	+	321	WP_047720740.1	hypothetical protein	-
AGF18_RS14395	3108558..3109919	+	1362	WP_047720739.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3082320..3122452	40132

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (7-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13644.59 Da
• Isoelectric Point: 9.7700

>T68362 WP_165569689.1 NZ_CP017928:3105105-3105473 [Klebsiella oxytoca]
IQSLPPQRAASSRLSSVKIWQKLLEYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGFSV
KEQSPFIGSLDMLRDRKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 369 bp

>T68362 NZ_CP017928:3105105-3105473 [Klebsiella oxytoca]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCAAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAACACATTGATGCGG
GAATCTCACTGTGTGACGCTGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGACCGGAAGGCTACTGGGCTGATGACCCGCAAGGGATA
TAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13394.53 Da
• Isoelectric Point: 8.1441

>AT68362 WP_047720742.1 NZ_CP017928:3104701-3105060 [Klebsiella oxytoca]
MQKATRVINHNIAEPWWGLRRNVTPCFGARLVQEGNRLHYLADRASTTGTFNDADLRHLDQAFPLLMKQMELMLTSSELM
PRIQRCITLHAKGLICEADTLGSCGYLYIVIYPASATTA

Nucleotide

• Length: 360 bp

>AT68362 NZ_CP017928:3104701-3105060 [Klebsiella oxytoca]
ATGCAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACGTCACGCCATGTTT
TGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCACCACCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGTTACTGATGAAGCAGATGGAGCTCATGCTGACCAGCAGCGAACTCATG
CCCCGCATTCAGCGCTGCATCACGCTCCATGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGTTCCTGTGGCTATTT
GTACATCGTAATTTATCCGGCGTCTGCAACAACCGCATAA