Detailed information of TA system TA068341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4083070..4083744
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: BK419_RS19070
• Protein ID: WP_003417282.1
• Coordinates: 4083316..4083744 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: BK419_RS19065
• Protein ID: WP_003417286.1
• Coordinates: 4083070..4083312 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS19040	4079606..4080670	+	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-
BK419_RS19045	4080767..4081141	+	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
BK419_RS19050	4081138..4081671	+	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
BK419_RS19055	4081672..4082337	+	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
BK419_RS19060	4082334..4082948	+	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
BK419_RS19065	4083070..4083312	+	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
BK419_RS19070	4083316..4083744	+	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS19075	4083823..4084614	-	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
BK419_RS19080	4084614..4086386	-	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
BK419_RS19085	4086515..4086949	-	435	WP_003902441.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
BK419_RS19090	4086946..4087380	-	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
BK419_RS19095	4087521..4087922	+	402	WP_003417264.1	cytidine deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T68341 WP_003417282.1 NZ_CP017920:4083316-4083744 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T68341 NZ_CP017920:4083316-4083744 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT68341 WP_003417286.1 NZ_CP017920:4083070-4083312 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT68341 NZ_CP017920:4083070-4083312 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59