Detailed information of TA system TA068338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3954676..3955361
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: BK419_RS18585
• Protein ID: WP_003417998.1
• Coordinates: 3954676..3955086 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BK419_RS18590
• Protein ID: WP_180895138.1
• Coordinates: 3955083..3955361 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS18570	3950115..3951704	+	1590	WP_003901630.1	IMP dehydrogenase	-
BK419_RS18575	3951724..3952851	+	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
BK419_RS18580	3952907..3954643	+	1737	WP_003418002.1	cholesterol oxidase	-
BK419_RS18585	3954676..3955086	-	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS18590	3955083..3955361	-	279	WP_180895138.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BK419_RS18595	3955417..3956304	-	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
BK419_RS18600	3956366..3956932	+	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
BK419_RS18605	3957050..3957754	+	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
BK419_RS18610	3957771..3959372	+	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-58)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T68338 WP_003417998.1 NZ_CP017920:c3955086-3954676 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T68338 NZ_CP017920:c3955086-3954676 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10227.70 Da
• Isoelectric Point: 8.0222

>AT68338 WP_180895138.1 NZ_CP017920:c3955361-3955083 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGCKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT68338 NZ_CP017920:c3955361-3955083 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCTGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: G0TIR9