Detailed information of TA system TA068335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 3009294..3009865
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: BK419_RS14335
• Protein ID: WP_003401560.1
• Coordinates: 3009563..3009865 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: BK419_RS14330
• Protein ID: WP_003401563.1
• Coordinates: 3009294..3009515 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS14315	3007199..3008107	-	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-
BK419_RS14320	3008104..3008736	-	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
BK419_RS14325	3008872..3009297	-	426	WP_003401566.1	PIN domain nuclease	-
BK419_RS14330	3009294..3009515	-	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
BK419_RS14335	3009563..3009865	-	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
BK419_RS14340	3009862..3010089	-	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
BK419_RS14345	3010232..3012007	-	1776	WP_010886074.1	PE family protein	-
BK419_RS14350	3012186..3013583	+	1398	WP_003401544.1	sulfatase	-
BK419_RS14355	3013593..3014396	+	804	WP_003401540.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T68335 WP_003401560.1 NZ_CP017920:c3009865-3009563 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T68335 NZ_CP017920:c3009865-3009563 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT68335 WP_003401563.1 NZ_CP017920:c3009515-3009294 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT68335 NZ_CP017920:c3009515-3009294 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48