68330

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 2677884..2678530 Replicon chromosome
Accession NZ_CP017920
Organism Mycobacterium tuberculosis strain TB282

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag BK419_RS12825 Protein ID WP_003403122.1
Coordinates 2678138..2678530 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag BK419_RS12820 Protein ID WP_003403125.1
Coordinates 2677884..2678141 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK419_RS12785 2673202..2673513 - 312 WP_003403164.1 hypothetical protein -
BK419_RS12790 2673570..2674331 + 762 WP_003403148.1 two-component system response regulator TcrA -
BK419_RS12795 2674315..2674845 + 531 WP_003917320.1 two component system sensor kinase HK2 -
BK419_RS12800 2674959..2675465 + 507 WP_003900973.1 two component system sensor kinase HK1 -
BK419_RS12805 2675569..2675805 + 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BK419_RS12810 2675802..2676215 + 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
BK419_RS12815 2676466..2677701 + 1236 WP_003403128.1 ATP-binding protein -
BK419_RS12820 2677884..2678141 + 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
BK419_RS12825 2678138..2678530 + 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
BK419_RS12830 2678582..2680132 - 1551 WP_003905349.1 MCE family protein -
BK419_RS12835 2680137..2681279 - 1143 WP_003911253.1 virulence factor Mce family protein -
BK419_RS12840 2681342..2682868 - 1527 WP_003403112.1 virulence factor Mce family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T68330 WP_003403122.1 NZ_CP017920:2678138-2678530 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T68330 NZ_CP017920:2678138-2678530 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT68330 WP_003403125.1 NZ_CP017920:2677884-2678141 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT68330 NZ_CP017920:2677884-2678141 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References