Detailed information of TA system TA068316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2132925..2133493
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BK419_RS10010
• Protein ID: WP_003405865.1
• Coordinates: 2132925..2133299 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BK419_RS10015
• Protein ID: WP_003405863.1
• Coordinates: 2133296..2133493 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS09975	2129338..2130045	+	708	Protein_1957	adenylate/guanylate cyclase domain-containing protein	-
BK419_RS22525	2129997..2130206	-	210	WP_003911400.1	hypothetical protein	-
BK419_RS09985	2130228..2130938	+	711	WP_003911399.1	hypothetical protein	-
BK419_RS09990	2130953..2131276	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BK419_RS09995	2131519..2131794	+	276	WP_003405867.1	hypothetical protein	-
BK419_RS10000	2131721..2131906	-	186	WP_003901093.1	hypothetical protein	-
BK419_RS10005	2132024..2132722	-	699	WP_003898733.1	hypothetical protein	-
BK419_RS10010	2132925..2133299	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS10015	2133296..2133493	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BK419_RS10020	2133581..2134654	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BK419_RS10025	2134717..2135700	+	984	WP_003898731.1	hypothetical protein	-
BK419_RS10030	2135717..2136724	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BK419_RS10035	2136814..2137452	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T68316 WP_003405865.1 NZ_CP017920:c2133299-2132925 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T68316 NZ_CP017920:c2133299-2132925 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT68316 WP_003405863.1 NZ_CP017920:c2133493-2133296 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT68316 NZ_CP017920:c2133493-2133296 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07