Detailed information of TA system TA068315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1989427..1990115
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: BK419_RS09340
• Protein ID: WP_003406304.1
• Coordinates: 1989427..1989858 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: BK419_RS09345
• Protein ID: WP_003406302.1
• Coordinates: 1989855..1990115 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS09315	1985254..1985523	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BK419_RS09320	1985520..1985813	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
BK419_RS09325	1985870..1986700	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
BK419_RS09330	1986781..1987641	-	861	WP_003900301.1	hypothetical protein	-
BK419_RS09335	1987821..1989404	+	1584	WP_009940130.1	PE family protein	-
BK419_RS09340	1989427..1989858	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS09345	1989855..1990115	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
BK419_RS09350	1990191..1991180	-	990	WP_003406301.1	malate dehydrogenase	-
BK419_RS09355	1991351..1992451	+	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
BK419_RS09360	1992528..1993709	-	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
BK419_RS09365	1993714..1994538	-	825	WP_003900298.1	trehalose ABC transporter permease SugB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T68315 WP_003406304.1 NZ_CP017920:c1989858-1989427 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T68315 NZ_CP017920:c1989858-1989427 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT68315 WP_003406302.1 NZ_CP017920:c1990115-1989855 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT68315 NZ_CP017920:c1990115-1989855 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6