Detailed information of TA system TA068313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1833470..1834520
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: G0TIH2
• Locus tag: BK419_RS08670
• Protein ID: WP_003407164.1
• Coordinates: 1833699..1834520 (-)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: BK419_RS08665
• Protein ID: WP_003407167.1
• Coordinates: 1833470..1833706 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS08645	1828992..1830253	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
BK419_RS08650	1830295..1830909	-	615	Protein_1702	cytochrome b5 domain-containing protein	-
BK419_RS08655	1831105..1831890	-	786	WP_003902048.1	lipoprotein LprF	-
BK419_RS08660	1832193..1833398	+	1206	WP_003918234.1	serine hydrolase	-
BK419_RS08665	1833470..1833706	-	237	WP_003407167.1	hypothetical protein	Antitoxin
BK419_RS08670	1833699..1834520	-	822	WP_003407164.1	GTP pyrophosphokinase family protein	Toxin
BK419_RS08675	1834741..1835127	+	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
BK419_RS08680	1835266..1837227	+	1962	WP_003407159.1	sigma-F factor regulator	-
BK419_RS08685	1837518..1838303	+	786	WP_003407157.1	membrane protein	-
BK419_RS08690	1838300..1838962	+	663	WP_003407152.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30100.10 Da
• Isoelectric Point: 5.8187

>T68313 WP_003407164.1 NZ_CP017920:c1834520-1833699 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADLLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T68313 NZ_CP017920:c1834520-1833699 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT68313 WP_003407167.1 NZ_CP017920:c1833706-1833470 [Mycobacterium tuberculosis]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT68313 NZ_CP017920:c1833706-1833470 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: G0TIH2

Antitoxin

• AlphaFold DB: A0A7U4BTV8