Detailed information of TA system TA068301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1122314..1122940
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BK419_RS05385
• Protein ID: WP_003410075.1
• Coordinates: 1122314..1122712 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: BK419_RS05390
• Protein ID: WP_003911750.1
• Coordinates: 1122713..1122940 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS05355	1117359..1117559	-	201	Protein_1045	hypothetical protein	-
BK419_RS05360	1117913..1119169	+	1257	WP_003902247.1	HNH endonuclease	-
BK419_RS05365	1119297..1119887	-	591	WP_003899131.1	IS110 family transposase	-
BK419_RS05370	1119841..1120488	-	648	WP_071854215.1	transposase	-
BK419_RS21125	1120549..1120728	+	180	Protein_1049	hypothetical protein	-
BK419_RS05375	1121165..1121599	-	435	WP_192931687.1	DUF1398 domain-containing protein	-
BK419_RS05380	1121700..1122131	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BK419_RS05385	1122314..1122712	-	399	WP_003410075.1	PIN domain nuclease	Toxin
BK419_RS05390	1122713..1122940	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BK419_RS05395	1123199..1124368	+	1170	WP_003899126.1	ATP-binding protein	-
BK419_RS05400	1124557..1124901	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T68301 WP_003410075.1 NZ_CP017920:c1122712-1122314 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T68301 NZ_CP017920:c1122712-1122314 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT68301 WP_003911750.1 NZ_CP017920:c1122940-1122713 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT68301 NZ_CP017920:c1122940-1122713 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8