Detailed information of TA system TA068298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1009837..1010532
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BK419_RS04875
• Protein ID: WP_003905754.1
• Coordinates: 1010098..1010532 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TMN0
• Locus tag: BK419_RS04870
• Protein ID: WP_003410814.1
• Coordinates: 1009837..1010091 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS04845	1005637..1006898	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
BK419_RS04850	1006963..1007259	-	297	WP_003410820.1	PE family protein	-
BK419_RS04865	1009564..1009821	+	258	WP_003410816.1	hypothetical protein	-
BK419_RS04870	1009837..1010091	+	255	WP_003410814.1	antitoxin	Antitoxin
BK419_RS04875	1010098..1010532	+	435	WP_003905754.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS04880	1010511..1011344	-	834	WP_003899172.1	SWIM zinc finger family protein	-
BK419_RS04885	1011337..1014378	-	3042	WP_003899171.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1004817..1017887	13070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15632.05 Da
• Isoelectric Point: 7.4681

>T68298 WP_003905754.1 NZ_CP017920:1010098-1010532 [Mycobacterium tuberculosis]
MKIVDANVLLYAVNTASEHHKPSLRWLDGALSGADRVGFAWVPLLAFVRLATKVGLFPRPLPREAAITQVADWLAAPSAV
LVNPTVRHADILARMLTYVGTGANLVNDAHLAALAVEHRASIVSYDSDFGRFEGVRWDQPPALL

Nucleotide

• Length: 435 bp

>T68298 NZ_CP017920:1010098-1010532 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCGCAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9417.57 Da
• Isoelectric Point: 8.9912

>AT68298 WP_003410814.1 NZ_CP017920:1009837-1010091 [Mycobacterium tuberculosis]
MRTTVTLDDDVEQLVRRRMAERQVSFKKALNDAIRDGASGRPAPSHFSTRTADLGVPAVNLDRALQLAADLEDEELVRRQ
RRGS

Nucleotide

• Length: 255 bp

>AT68298 NZ_CP017920:1009837-1010091 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Antitoxin

• AlphaFold DB: G0TMN0