Detailed information of TA system TA068295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 521507..522147
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BK419_RS02550
• Protein ID: WP_003412970.1
• Coordinates: 521728..522147 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BK419_RS02545
• Protein ID: WP_003412975.1
• Coordinates: 521507..521731 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS02525	517124..517687	+	564	WP_003412989.1	elongation factor P	-
BK419_RS02530	517690..518160	+	471	WP_003899364.1	transcription antitermination factor NusB	-
BK419_RS02535	518160..518561	+	402	WP_003412981.1	hypothetical protein	-
BK419_RS02540	518633..521476	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BK419_RS02545	521507..521731	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BK419_RS02550	521728..522147	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS02555	522148..523098	-	951	WP_003911916.1	Lsr2 family protein	-
BK419_RS22400	523157..523402	+	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BK419_RS02560	523743..524663	+	921	WP_003412965.1	restriction endonuclease	-
BK419_RS02565	524698..525099	-	402	WP_003412963.1	PIN domain-containing protein	-
BK419_RS02570	525096..525318	-	223	Protein_512	type II toxin-antitoxin system VapB family antitoxin	-
BK419_RS02575	525835..526557	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T68295 WP_003412970.1 NZ_CP017920:521728-522147 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T68295 NZ_CP017920:521728-522147 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT68295 WP_003412975.1 NZ_CP017920:521507-521731 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT68295 NZ_CP017920:521507-521731 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09