Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 506050..506687 Replicon chromosome
Accession NZ_CP017920
Organism Mycobacterium tuberculosis strain TB282

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BK419_RS02445 Protein ID WP_003413180.1
Coordinates 506292..506687 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BK419_RS02440 Protein ID WP_003413183.1
Coordinates 506050..506295 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK419_RS02420 502644..503156 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
BK419_RS02425 503149..504402 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
BK419_RS02430 504399..505208 + 810 WP_003413193.1 shikimate dehydrogenase -
BK419_RS02435 505220..505639 + 420 WP_003413190.1 A24 family peptidase -
BK419_RS02440 506050..506295 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BK419_RS02445 506292..506687 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BK419_RS02450 506787..507161 + 375 WP_003413177.1 hypothetical protein -
BK419_RS02455 507177..507554 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BK419_RS02460 507551..507808 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BK419_RS02465 507847..508260 - 414 WP_003413164.1 PIN domain nuclease -
BK419_RS22120 508353..508630 - 278 Protein_491 type II toxin-antitoxin system VapB family antitoxin -
BK419_RS02475 508627..509289 - 663 WP_003905878.1 hypothetical protein -
BK419_RS02480 509286..509945 - 660 WP_003902296.1 hypothetical protein -
BK419_RS02485 510072..511283 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T68292 WP_003413180.1 NZ_CP017920:506292-506687 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T68292 NZ_CP017920:506292-506687 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT68292 WP_003413183.1 NZ_CP017920:506050-506295 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT68292 NZ_CP017920:506050-506295 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References