Detailed information of TA system TA068288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 306255..306942
• Replicon: chromosome
• Accession: NZ_CP017920
• Organism: Mycobacterium tuberculosis strain TB282

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: BK419_RS01365
• Protein ID: WP_003414064.1
• Coordinates: 306255..306650 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BK419_RS01370
• Protein ID: WP_003414061.1
• Coordinates: 306676..306942 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK419_RS01335	302152..302889	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
BK419_RS01340	303045..303845	+	801	WP_003911953.1	thymidylate synthase	-
BK419_RS01345	303916..304395	+	480	WP_003414073.1	dihydrofolate reductase	-
BK419_RS01350	304469..304888	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
BK419_RS01355	304885..305979	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
BK419_RS01360	305989..306258	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BK419_RS01365	306255..306650	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK419_RS01370	306676..306942	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BK419_RS01375	306939..307355	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
BK419_RS01380	307442..309064	+	1623	WP_003414057.1	type I restriction-modification system subunit M	-
BK419_RS01385	309061..309336	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BK419_RS01390	309580..310332	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BK419_RS01395	310401..311303	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T68288 WP_003414064.1 NZ_CP017920:306255-306650 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T68288 NZ_CP017920:306255-306650 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT68288 WP_003414061.1 NZ_CP017920:306676-306942 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT68288 NZ_CP017920:306676-306942 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64