Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 266788..267358 Replicon chromosome
Accession NZ_CP017920
Organism Mycobacterium tuberculosis strain TB282

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag BK419_RS01155 Protein ID WP_003414166.1
Coordinates 267002..267358 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag BK419_RS01150 Protein ID WP_003901465.1
Coordinates 266788..267018 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK419_RS01110 261807..262118 - 312 WP_003414190.1 hypothetical protein -
BK419_RS01115 262223..262480 - 258 WP_003899489.1 hypothetical protein -
BK419_RS01120 262713..264002 - 1290 Protein_221 transposase family protein -
BK419_RS01125 264066..264257 - 192 WP_003414184.1 hypothetical protein -
BK419_RS01130 264254..264658 - 405 WP_003414181.1 hypothetical protein -
BK419_RS22100 264806..265060 + 255 WP_003917684.1 hypothetical protein -
BK419_RS22105 265236..265511 - 276 WP_003911993.1 DUF1778 domain-containing protein -
BK419_RS01145 265702..266745 + 1044 WP_003414172.1 DUF2293 domain-containing protein -
BK419_RS01150 266788..267018 + 231 WP_003901465.1 antitoxin MazE Antitoxin
BK419_RS01155 267002..267358 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
BK419_RS01160 267460..269109 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
BK419_RS01165 269128..269715 - 588 WP_003914429.1 DUF3558 family protein -
BK419_RS01170 269888..270214 + 327 WP_003414157.1 hypothetical protein -
BK419_RS01175 270218..271906 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T68287 WP_003414166.1 NZ_CP017920:267002-267358 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T68287 NZ_CP017920:267002-267358 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT68287 WP_003901465.1 NZ_CP017920:266788-267018 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT68287 NZ_CP017920:266788-267018 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References