Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 246557..247161 Replicon chromosome
Accession NZ_CP017920
Organism Mycobacterium tuberculosis strain TB282

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag BK419_RS01025 Protein ID WP_003414492.1
Coordinates 246769..247161 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag BK419_RS01020 Protein ID WP_003414495.1
Coordinates 246557..246772 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK419_RS00995 241559..242470 + 912 WP_003414505.1 sugar ABC transporter permease -
BK419_RS01000 242467..243294 + 828 WP_003414504.1 carbohydrate ABC transporter permease -
BK419_RS01005 243297..244607 + 1311 WP_003414501.1 ABC transporter substrate-binding protein -
BK419_RS01010 244600..245682 + 1083 WP_003414499.1 ABC transporter ATP-binding protein -
BK419_RS01015 245761..246510 - 750 WP_003902358.1 enoyl-CoA hydratase -
BK419_RS01020 246557..246772 + 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BK419_RS01025 246769..247161 + 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
BK419_RS01030 247182..247451 + 270 WP_003414489.1 DUF2277 family protein -
BK419_RS01035 247448..247993 + 546 WP_003910939.1 DUF1802 family protein -
BK419_RS01040 248298..249185 + 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
BK419_RS01045 249188..250072 + 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
BK419_RS01050 250344..250889 + 546 WP_014584866.1 DUF1802 family protein -
BK419_RS01055 251223..252011 + 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T68285 WP_003414492.1 NZ_CP017920:246769-247161 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T68285 NZ_CP017920:246769-247161 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT68285 WP_003414495.1 NZ_CP017920:246557-246772 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT68285 NZ_CP017920:246557-246772 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References