Detailed information of TA system TA068280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1936819..1937347
• Replicon: chromosome
• Accession: NZ_CP017916
• Organism: Vibrio alginolyticus strain K08M4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K08M4_RS08830
• Protein ID: WP_086049614.1
• Coordinates: 1937057..1937347 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K5UYY9
• Locus tag: K08M4_RS08825
• Protein ID: WP_004730320.1
• Coordinates: 1936819..1937067 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K08M4_RS08780	1932520..1933248	-	729	WP_086049612.1	glycosyltransferase	-
K08M4_RS08785	1933618..1933896	+	279	WP_009847093.1	50S ribosomal protein L25	-
K08M4_RS08790	1934346..1934672	-	327	WP_086049613.1	hypothetical protein	-
K08M4_RS08795	1934774..1935054	-	281	Protein_1645	hypothetical protein	-
K08M4_RS08800	1935262..1935483	+	222	WP_009847096.1	hypothetical protein	-
K08M4_RS08805	1935476..1935766	+	291	WP_009847097.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K08M4_RS08815	1936229..1936603	-	375	WP_086050413.1	VOC family protein	-
K08M4_RS08820	1936676..1936744	-	69	WP_077672877.1	DUF3265 domain-containing protein	-
K08M4_RS08825	1936819..1937067	+	249	WP_004730320.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K08M4_RS08830	1937057..1937347	+	291	WP_086049614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K08M4_RS08835	1937444..1937509	-	66	WP_198299329.1	DUF3265 domain-containing protein	-
K08M4_RS08840	1937619..1938506	+	888	WP_017080519.1	site-specific integrase	-
K08M4_RS08845	1938503..1939461	+	959	Protein_1654	transposase	-
K08M4_RS08850	1939725..1941179	-	1455	WP_086049615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1936229..1958427	22198
	-	flank	IS/Tn	-	-	1937619..1938506	887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11219.13 Da
• Isoelectric Point: 10.5339

>T68280 WP_086049614.1 NZ_CP017916:1937057-1937347 [Vibrio alginolyticus]
MTYKLDFKKSALKEWKKLGSTLQQQFKKKLIERLDNPHVPASKLSGADNMYKIKLRQSGYRLVYNVEDDVIIVTVLAVGK
RERSDAYRKAMKRLDD

Nucleotide

• Length: 291 bp

>T68280 NZ_CP017916:1937057-1937347 [Vibrio alginolyticus]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCAACGCTTCAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCGCATGTTCCGGCTTCAAAACTCTCTGGAGCTGACAACATGTATAAGATTA
AGCTTCGCCAATCGGGCTACCGTCTCGTCTACAACGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCTGTCGGAAAA
CGCGAACGTAGCGATGCTTACCGTAAAGCTATGAAAAGGTTAGATGATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9109.39 Da
• Isoelectric Point: 3.9610

>AT68280 WP_004730320.1 NZ_CP017916:1936819-1937067 [Vibrio alginolyticus]
MTTRILADVAASITELKANPMKVATSAYGEPVAVLNRNEPAFYCVPAEAYEMMMDRLEDLELLTIAKERESEESISVNID
DL

Nucleotide

• Length: 249 bp

>AT68280 NZ_CP017916:1936819-1937067 [Vibrio alginolyticus]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACTGAATTAAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTACGGTGAACCTGTTGCTGTACTGAACCGAAATGAGCCAGCATTTTATTGCGTACCAGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTAACTATTGCGAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0L1LA33