Detailed information of TA system TA068279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 1646320..1646867
• Replicon: chromosome
• Accession: NZ_CP017916
• Organism: Vibrio alginolyticus strain K08M4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K08M4_RS07690
• Protein ID: WP_086049456.1
• Coordinates: 1646320..1646622 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1B1BV57
• Locus tag: K08M4_RS07695
• Protein ID: WP_016787072.1
• Coordinates: 1646610..1646867 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K08M4_RS07670	1641557..1641922	+	366	WP_086049453.1	hypothetical protein	-
K08M4_RS07675	1642038..1643222	+	1185	WP_086049454.1	FIST C-terminal domain-containing protein	-
K08M4_RS07680	1643222..1644946	+	1725	WP_086049455.1	response regulator	-
K08M4_RS07685	1645218..1646120	+	903	Protein_1427	ISNCY family transposase	-
K08M4_RS07690	1646320..1646622	-	303	WP_086049456.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K08M4_RS07695	1646610..1646867	-	258	WP_016787072.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K08M4_RS07700	1647039..1647641	-	603	WP_086049457.1	hypothetical protein	-
K08M4_RS07705	1647653..1648012	-	360	WP_086049458.1	mCpol domain-containing protein	-
K08M4_RS07710	1648203..1648838	-	636	WP_086049459.1	hypothetical protein	-
K08M4_RS07715	1648838..1649737	-	900	WP_086049460.1	nucleotidyltransferase	-
K08M4_RS07725	1650177..1650545	-	369	WP_086049462.1	hypothetical protein	-
K08M4_RS21960	1650715..1651750	+	1036	WP_157665735.1	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1642038..1651750	9712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11753.61 Da
• Isoelectric Point: 5.1972

>T68279 WP_086049456.1 NZ_CP017916:c1646622-1646320 [Vibrio alginolyticus]
MAEVIWSEPALSDLNDIAEYIALENLVAAKQLVQNIFDKIERLSDFPESGRLPPELEHLNYREVVVNPCRVFYKQKGDKV
YILFVMRAERDLRRFLLSKQ

Nucleotide

• Length: 303 bp

>T68279 NZ_CP017916:c1646622-1646320 [Vibrio alginolyticus]
ATGGCTGAAGTGATTTGGAGTGAACCCGCGCTATCGGATCTCAACGATATCGCGGAATACATTGCCCTTGAAAACCTAGT
CGCGGCCAAACAGCTGGTTCAAAACATTTTCGACAAAATTGAACGCCTCAGCGACTTTCCCGAGTCTGGGCGTCTGCCTC
CAGAGCTCGAGCACCTGAATTATCGCGAAGTCGTAGTGAATCCATGTCGGGTTTTCTACAAGCAAAAAGGTGACAAAGTG
TACATCTTGTTTGTGATGCGCGCGGAGCGAGATTTACGTCGGTTTTTGCTGAGCAAACAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9621.11 Da
• Isoelectric Point: 7.3177

>AT68279 WP_016787072.1 NZ_CP017916:c1646867-1646610 [Vibrio alginolyticus]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLVDVDDYQFMQNRLAILEGIARGERAISEGKALSHDEAKDKM
SKWLK

Nucleotide

• Length: 258 bp

>AT68279 NZ_CP017916:c1646867-1646610 [Vibrio alginolyticus]
ATGAAAGTCGAGCTCGTTACTTCCCTTAAGCGTCAAGCGACCAAAATCCTTGCCGACCTACACGACACCAAAGAGCCCGT
GTTGATCACCGAGCATGGCAAACCTTCTGCGTATTTGGTTGACGTCGATGACTATCAGTTTATGCAAAACCGCTTGGCTA
TCCTCGAAGGGATCGCACGCGGTGAGCGCGCCATTTCTGAAGGCAAGGCGCTGAGTCATGACGAAGCCAAAGACAAGATG
TCAAAATGGCTGAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B1BV57