Detailed information of TA system TA068278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 38759..39287
• Replicon: plasmid pL300
• Accession: NZ_CP017915
• Organism: Vibrio alginolyticus strain K08M3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1W6UUU0
• Locus tag: K08M3_RS25290
• Protein ID: WP_025767362.1
• Coordinates: 39003..39287 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K08M3_RS25285
• Protein ID: WP_086048372.1
• Coordinates: 38759..39013 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K08M3_RS25250	34088..34462	-	375	WP_025767354.1	hypothetical protein	-
K08M3_RS25255	34643..35473	-	831	WP_086048366.1	hypothetical protein	-
K08M3_RS26945	35620..35796	-	177	WP_157664648.1	hypothetical protein	-
K08M3_RS25260	35807..36400	-	594	WP_086048367.1	hypothetical protein	-
K08M3_RS25265	36611..36949	+	339	WP_086048368.1	hypothetical protein	-
K08M3_RS25270	36998..37231	-	234	WP_086048369.1	hypothetical protein	-
K08M3_RS25275	37250..37684	-	435	WP_086048370.1	hypothetical protein	-
K08M3_RS25280	37750..38502	-	753	WP_086048371.1	hypothetical protein	-
K08M3_RS25285	38759..39013	+	255	WP_086048372.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K08M3_RS25290	39003..39287	+	285	WP_025767362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K08M3_RS25300	39524..39712	+	189	WP_025767364.1	hypothetical protein	-
K08M3_RS25305	39725..39988	+	264	WP_025767365.1	hypothetical protein	-
K08M3_RS25310	40223..40543	-	321	WP_086048373.1	hypothetical protein	-
K08M3_RS25315	40795..41541	+	747	WP_025767367.1	hypothetical protein	-
K08M3_RS25320	42776..43084	+	309	WP_086048374.1	hypothetical protein	-
K08M3_RS25325	43382..43702	+	321	WP_086048375.1	hypothetical protein	-
K08M3_RS25330	43712..43915	+	204	WP_025767370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..300425	300425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11103.10 Da
• Isoelectric Point: 10.6691

>T68278 WP_025767362.1 NZ_CP017915:39003-39287 [Vibrio alginolyticus]
MTYKLVFKTEAEKEWRKLDSTIKSQFKKKLIERLENPHVPTAKLSSMENCYKIKLRSAGYRLVYQVRDQEVIISVVAVGK
RERNQVYKIAAKRV

Nucleotide

• Length: 285 bp

>T68278 NZ_CP017915:39003-39287 [Vibrio alginolyticus]
ATGACTTATAAGTTAGTTTTCAAGACTGAGGCAGAGAAAGAATGGCGCAAGTTGGACAGCACTATTAAGTCTCAGTTTAA
GAAGAAGCTGATTGAACGTTTAGAAAATCCGCATGTACCAACGGCAAAATTAAGCAGTATGGAGAATTGCTACAAAATAA
AACTGAGAAGTGCAGGATACCGGCTTGTTTATCAAGTGAGAGATCAGGAAGTAATTATTTCAGTTGTGGCAGTTGGTAAG
CGAGAACGTAATCAGGTTTATAAAATCGCTGCAAAAAGAGTGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9474.81 Da
• Isoelectric Point: 3.9953

>AT68278 WP_086048372.1 NZ_CP017915:38759-39013 [Vibrio alginolyticus]
MPTIRPILADNSTSISELKKNPMAVIEQADGFPVAVLNRNQPAFYCVPADAYEMLMDKLEDIELAQIVKEREHSEEIEIS
IDDL

Nucleotide

• Length: 255 bp

>AT68278 NZ_CP017915:38759-39013 [Vibrio alginolyticus]
ATGCCAACAATTCGTCCTATTCTCGCTGATAATTCAACAAGTATCAGCGAACTTAAAAAGAACCCTATGGCAGTCATAGA
ACAAGCTGACGGGTTCCCTGTTGCGGTACTGAACCGTAATCAACCTGCATTTTACTGCGTTCCTGCGGATGCCTATGAAA
TGCTCATGGATAAACTGGAAGATATTGAGCTTGCTCAGATAGTTAAAGAGCGTGAGCATTCTGAAGAAATAGAGATCAGC
ATCGATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A1W6UUU0