Detailed information of TA system TA068273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 38759..39287
• Replicon: plasmid pL289
• Accession: NZ_CP017904
• Organism: Vibrio alginolyticus strain K05K4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1W6UUU0
• Locus tag: K05K4_RS25475
• Protein ID: WP_025767362.1
• Coordinates: 39003..39287 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K05K4_RS25470
• Protein ID: WP_086048372.1
• Coordinates: 38759..39013 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K05K4_RS25435	34088..34462	-	375	WP_025767354.1	hypothetical protein	-
K05K4_RS25440	34643..35473	-	831	WP_086048366.1	hypothetical protein	-
K05K4_RS25445	35807..36400	-	594	WP_086048367.1	hypothetical protein	-
K05K4_RS25450	36611..36949	+	339	WP_086048368.1	hypothetical protein	-
K05K4_RS25455	36998..37231	-	234	WP_086048369.1	hypothetical protein	-
K05K4_RS25460	37250..37684	-	435	WP_086048370.1	hypothetical protein	-
K05K4_RS25465	37750..38502	-	753	WP_086048371.1	hypothetical protein	-
K05K4_RS25470	38759..39013	+	255	WP_086048372.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K05K4_RS25475	39003..39287	+	285	WP_025767362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K05K4_RS25480	39302..39505	+	204	WP_025767363.1	hypothetical protein	-
K05K4_RS25485	39524..39712	+	189	WP_025767364.1	hypothetical protein	-
K05K4_RS25490	39725..39988	+	264	WP_025767365.1	hypothetical protein	-
K05K4_RS25495	40223..40543	-	321	WP_086048373.1	hypothetical protein	-
K05K4_RS25500	40795..41541	+	747	WP_025767367.1	hypothetical protein	-
K05K4_RS25505	42776..43084	+	309	WP_086048374.1	hypothetical protein	-
K05K4_RS25510	43382..43702	+	321	WP_086048375.1	hypothetical protein	-
K05K4_RS25515	43712..43915	+	204	WP_025767370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..289065	289065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11103.10 Da
• Isoelectric Point: 10.6691

>T68273 WP_025767362.1 NZ_CP017904:39003-39287 [Vibrio alginolyticus]
MTYKLVFKTEAEKEWRKLDSTIKSQFKKKLIERLENPHVPTAKLSSMENCYKIKLRSAGYRLVYQVRDQEVIISVVAVGK
RERNQVYKIAAKRV

Nucleotide

• Length: 285 bp

>T68273 NZ_CP017904:39003-39287 [Vibrio alginolyticus]
ATGACTTATAAGTTAGTTTTCAAGACTGAGGCAGAGAAAGAATGGCGCAAGTTGGACAGCACTATTAAGTCTCAGTTTAA
GAAGAAGCTGATTGAACGTTTAGAAAATCCGCATGTACCAACGGCAAAATTAAGCAGTATGGAGAATTGCTACAAAATAA
AACTGAGAAGTGCAGGATACCGGCTTGTTTATCAAGTGAGAGATCAGGAAGTAATTATTTCAGTTGTGGCAGTTGGTAAG
CGAGAACGTAATCAGGTTTATAAAATCGCTGCAAAAAGAGTGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9474.81 Da
• Isoelectric Point: 3.9953

>AT68273 WP_086048372.1 NZ_CP017904:38759-39013 [Vibrio alginolyticus]
MPTIRPILADNSTSISELKKNPMAVIEQADGFPVAVLNRNQPAFYCVPADAYEMLMDKLEDIELAQIVKEREHSEEIEIS
IDDL

Nucleotide

• Length: 255 bp

>AT68273 NZ_CP017904:38759-39013 [Vibrio alginolyticus]
ATGCCAACAATTCGTCCTATTCTCGCTGATAATTCAACAAGTATCAGCGAACTTAAAAAGAACCCTATGGCAGTCATAGA
ACAAGCTGACGGGTTCCCTGTTGCGGTACTGAACCGTAATCAACCTGCATTTTACTGCGTTCCTGCGGATGCCTATGAAA
TGCTCATGGATAAACTGGAAGATATTGAGCTTGCTCAGATAGTTAAAGAGCGTGAGCATTCTGAAGAAATAGAGATCAGC
ATCGATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A1W6UUU0