Detailed information of TA system TA068271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 60821..61349
• Replicon: plasmid pL294
• Accession: NZ_CP017901
• Organism: Vibrio alginolyticus strain K04M5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1W6UUU0
• Locus tag: K04M5_RS25430
• Protein ID: WP_025767362.1
• Coordinates: 61065..61349 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K04M5_RS25425
• Protein ID: WP_086048372.1
• Coordinates: 60821..61075 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K04M5_RS25390	56150..56524	-	375	WP_025767354.1	hypothetical protein	-
K04M5_RS25395	56705..57535	-	831	WP_086048366.1	hypothetical protein	-
K04M5_RS26945	57682..57858	-	177	WP_157664648.1	hypothetical protein	-
K04M5_RS25400	57869..58462	-	594	WP_086048367.1	hypothetical protein	-
K04M5_RS25405	58673..59011	+	339	WP_086048368.1	hypothetical protein	-
K04M5_RS25410	59060..59293	-	234	WP_086048369.1	hypothetical protein	-
K04M5_RS25415	59312..59746	-	435	WP_086048370.1	hypothetical protein	-
K04M5_RS25420	59812..60564	-	753	WP_086048371.1	hypothetical protein	-
K04M5_RS25425	60821..61075	+	255	WP_086048372.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K04M5_RS25430	61065..61349	+	285	WP_025767362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K04M5_RS25440	61586..61774	+	189	WP_025767364.1	hypothetical protein	-
K04M5_RS25445	61787..62050	+	264	WP_025767365.1	hypothetical protein	-
K04M5_RS25450	62285..62605	-	321	WP_086048373.1	hypothetical protein	-
K04M5_RS25455	62857..63603	+	747	WP_025767367.1	hypothetical protein	-
K04M5_RS25460	64838..65146	+	309	WP_086048374.1	hypothetical protein	-
K04M5_RS25465	65444..65764	+	321	WP_086048375.1	hypothetical protein	-
K04M5_RS25470	65774..65977	+	204	WP_025767370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..294721	294721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11103.10 Da
• Isoelectric Point: 10.6691

>T68271 WP_025767362.1 NZ_CP017901:61065-61349 [Vibrio alginolyticus]
MTYKLVFKTEAEKEWRKLDSTIKSQFKKKLIERLENPHVPTAKLSSMENCYKIKLRSAGYRLVYQVRDQEVIISVVAVGK
RERNQVYKIAAKRV

Nucleotide

• Length: 285 bp

>T68271 NZ_CP017901:61065-61349 [Vibrio alginolyticus]
ATGACTTATAAGTTAGTTTTCAAGACTGAGGCAGAGAAAGAATGGCGCAAGTTGGACAGCACTATTAAGTCTCAGTTTAA
GAAGAAGCTGATTGAACGTTTAGAAAATCCGCATGTACCAACGGCAAAATTAAGCAGTATGGAGAATTGCTACAAAATAA
AACTGAGAAGTGCAGGATACCGGCTTGTTTATCAAGTGAGAGATCAGGAAGTAATTATTTCAGTTGTGGCAGTTGGTAAG
CGAGAACGTAATCAGGTTTATAAAATCGCTGCAAAAAGAGTGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9474.81 Da
• Isoelectric Point: 3.9953

>AT68271 WP_086048372.1 NZ_CP017901:60821-61075 [Vibrio alginolyticus]
MPTIRPILADNSTSISELKKNPMAVIEQADGFPVAVLNRNQPAFYCVPADAYEMLMDKLEDIELAQIVKEREHSEEIEIS
IDDL

Nucleotide

• Length: 255 bp

>AT68271 NZ_CP017901:60821-61075 [Vibrio alginolyticus]
ATGCCAACAATTCGTCCTATTCTCGCTGATAATTCAACAAGTATCAGCGAACTTAAAAAGAACCCTATGGCAGTCATAGA
ACAAGCTGACGGGTTCCCTGTTGCGGTACTGAACCGTAATCAACCTGCATTTTACTGCGTTCCTGCGGATGCCTATGAAA
TGCTCATGGATAAACTGGAAGATATTGAGCTTGCTCAGATAGTTAAAGAGCGTGAGCATTCTGAAGAAATAGAGATCAGC
ATCGATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A1W6UUU0