Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2592422..2593055 Replicon chromosome
Accession NZ_CP017888
Organism Weizmannia coagulans strain BC-HY1

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag BIZ35_RS12810 Protein ID WP_013860680.1
Coordinates 2592422..2592772 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag BIZ35_RS12815 Protein ID WP_014096808.1
Coordinates 2592777..2593055 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BIZ35_RS12770 2587678..2588457 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
BIZ35_RS12775 2588435..2588905 - 471 WP_014096814.1 anti-sigma B factor RsbW -
BIZ35_RS12780 2588909..2589244 - 336 WP_026104587.1 anti-sigma factor antagonist -
BIZ35_RS12785 2589314..2590324 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
BIZ35_RS12790 2590338..2590739 - 402 WP_014096811.1 anti-sigma regulatory factor -
BIZ35_RS12795 2590744..2591100 - 357 WP_014096810.1 STAS domain-containing protein -
BIZ35_RS12800 2591104..2591931 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
BIZ35_RS12810 2592422..2592772 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BIZ35_RS12815 2592777..2593055 - 279 WP_014096808.1 hypothetical protein Antitoxin
BIZ35_RS12820 2593208..2594359 - 1152 WP_014096807.1 alanine racemase -
BIZ35_RS12825 2594561..2595577 - 1017 WP_014096806.1 outer membrane lipoprotein carrier protein LolA -
BIZ35_RS12830 2595718..2596068 - 351 WP_014096805.1 holo-ACP synthase -
BIZ35_RS12835 2596349..2596948 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T68264 WP_013860680.1 NZ_CP017888:c2592772-2592422 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T68264 NZ_CP017888:c2592772-2592422 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT68264 WP_014096808.1 NZ_CP017888:c2593055-2592777 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT68264 NZ_CP017888:c2593055-2592777 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References