68258

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 2145544..2146140 Replicon chromosome
Accession NZ_CP017886
Organism Pseudomonas frederiksbergensis strain ERDD5:01

Toxin (Protein)


Gene name relE Uniprot ID A0A1J0EJJ9
Locus tag BLL42_RS10510 Protein ID WP_071551997.1
Coordinates 2145544..2145846 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A1J0EJU7
Locus tag BLL42_RS10515 Protein ID WP_071551998.1
Coordinates 2145850..2146140 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLL42_RS10490 2140731..2142038 - 1308 WP_071551993.1 precorrin-3B synthase -
BLL42_RS10495 2142315..2143526 + 1212 WP_071551994.1 precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE -
BLL42_RS10500 2143519..2144616 + 1098 WP_071551995.1 cobalt-precorrin-5B (C(1))-methyltransferase -
BLL42_RS10505 2144613..2145341 + 729 WP_071551996.1 cobalt-precorrin-6A reductase -
BLL42_RS10510 2145544..2145846 + 303 WP_071551997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BLL42_RS10515 2145850..2146140 + 291 WP_071551998.1 putative addiction module antidote protein Antitoxin
BLL42_RS10520 2146237..2146638 + 402 WP_071551999.1 DUF2946 domain-containing protein -
BLL42_RS10525 2146686..2147165 + 480 WP_071552000.1 copper chaperone PCu(A)C -
BLL42_RS10530 2147220..2147642 + 423 WP_071552001.1 DUF2946 domain-containing protein -
BLL42_RS10535 2147730..2149859 + 2130 WP_071552002.1 TonB-dependent copper receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-95)

Antitoxin

Uncharacterized protein HI1420

(5-94)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11408.94 Da        Isoelectric Point: 8.0382

>T68258 WP_071551997.1 NZ_CP017886:2145544-2145846 [Pseudomonas frederiksbergensis]
MIDFEQSRPFADWLHSLKDTIGKARIVARLRAAEHGNFGDCEAVGEAVYEMRVHYGPGYRMYFTRKGEVVYLLLAGGDKS
TQKRDIKRAQQMAQNIGNEE

Download         Length: 303 bp

>T68258 NZ_CP017886:2145544-2145846 [Pseudomonas frederiksbergensis]
ATGATCGACTTCGAACAGTCTCGACCATTTGCCGATTGGCTTCACTCACTCAAGGACACCATCGGCAAGGCTCGTATTGT
CGCCAGGCTTCGAGCTGCCGAGCATGGAAACTTCGGTGATTGTGAAGCAGTCGGTGAGGCTGTTTATGAAATGCGTGTGC
ATTACGGTCCCGGCTACAGGATGTACTTCACTCGTAAAGGTGAAGTGGTTTATCTGCTGTTGGCAGGAGGCGACAAGTCG
ACTCAAAAACGAGACATCAAACGCGCTCAACAGATGGCGCAAAACATCGGTAATGAGGAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10286.73 Da        Isoelectric Point: 4.5994

>AT68258 WP_071551998.1 NZ_CP017886:2145850-2146140 [Pseudomonas frederiksbergensis]
MAEQFTRFDAAEFLKTPEEMAAYLDACFEEDAGDGALIRAALNDIARAQGMTQVARDAGLGRESLYKALGSTGNPEFTTI
MKVMKALGLKLHATAA

Download         Length: 291 bp

>AT68258 NZ_CP017886:2145850-2146140 [Pseudomonas frederiksbergensis]
ATGGCCGAACAATTCACCCGTTTCGACGCCGCCGAATTTCTCAAAACCCCTGAGGAAATGGCGGCTTACCTGGATGCCTG
CTTTGAAGAAGATGCAGGTGACGGCGCTTTGATACGTGCCGCGCTCAACGACATCGCTCGGGCACAAGGCATGACGCAGG
TTGCCCGTGATGCAGGTTTAGGTCGTGAAAGTTTGTACAAGGCACTGGGCAGCACGGGTAATCCAGAATTCACGACTATC
ATGAAGGTGATGAAGGCCTTGGGGCTTAAGCTCCACGCAACAGCCGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1J0EJJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A1J0EJU7

References