Detailed information of TA system TA068257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1495143..1495656
• Replicon: chromosome
• Accession: NZ_CP017886
• Organism: Pseudomonas frederiksbergensis strain ERDD5:01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BLL42_RS07480
• Protein ID: WP_071551475.1
• Coordinates: 1495372..1495656 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1J0EI29
• Locus tag: BLL42_RS07475
• Protein ID: WP_071551474.1
• Coordinates: 1495143..1495382 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLL42_RS07445	1490950..1491453	+	504	WP_071551469.1	inclusion body family protein	-
BLL42_RS07450	1491576..1492115	+	540	WP_071551470.1	hypothetical protein	-
BLL42_RS07455	1492261..1492785	-	525	WP_071551471.1	DUF4142 domain-containing protein	-
BLL42_RS07460	1492917..1494260	-	1344	WP_071551472.1	GGDEF domain-containing protein	-
BLL42_RS07465	1494367..1494567	-	201	Protein_1447	LysE family translocator	-
BLL42_RS07470	1494603..1495016	-	414	WP_071551473.1	fosfomycin resistance glutathione transferase	-
BLL42_RS07475	1495143..1495382	+	240	WP_071551474.1	ribbon-helix-helix protein, CopG family	Antitoxin
BLL42_RS07480	1495372..1495656	+	285	WP_071551475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BLL42_RS07485	1495670..1496410	-	741	WP_071551476.1	hypothetical protein	-
BLL42_RS07490	1496505..1497653	-	1149	WP_071551477.1	L-threonine dehydrogenase	-
BLL42_RS07495	1497809..1498828	+	1020	WP_071555714.1	DUF4917 family protein	-
BLL42_RS07500	1498849..1499193	-	345	WP_071551478.1	YegP family protein	-
BLL42_RS07505	1499364..1499900	+	537	WP_071551479.1	RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10800.55 Da
• Isoelectric Point: 10.4208

>T68257 WP_071551475.1 NZ_CP017886:1495372-1495656 [Pseudomonas frederiksbergensis]
MRVEWLRTALKNIDDEAAYIALENPKAAADFVKTIFASVDHLAQFPAIGREGRLPGTREWVLTDRPYLIPYRVRQGRLQV
LRVFHTRRLPPGAC

Nucleotide

• Length: 285 bp

>T68257 NZ_CP017886:1495372-1495656 [Pseudomonas frederiksbergensis]
ATGCGCGTTGAGTGGCTGAGAACCGCACTAAAAAATATTGATGACGAAGCCGCTTACATCGCGCTGGAAAACCCGAAAGC
CGCCGCTGACTTCGTCAAAACCATCTTCGCCAGTGTCGACCACCTTGCCCAATTTCCTGCGATTGGCCGTGAAGGCCGTC
TGCCCGGTACCCGAGAGTGGGTACTAACTGACCGCCCCTACCTCATCCCTTATCGTGTTCGCCAAGGGCGTCTTCAGGTT
CTACGAGTGTTCCACACCCGCCGTCTACCACCCGGAGCATGCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8705.73 Da
• Isoelectric Point: 4.4354

>AT68257 WP_071551474.1 NZ_CP017886:1495143-1495382 [Pseudomonas frederiksbergensis]
MSVMSLRLPDEIADTLANLAKATGRSKSFLAVDALREYLAREAWQIEEIQHALKEADAGDFASDEEVNALANKWASNAR

Nucleotide

• Length: 240 bp

>AT68257 NZ_CP017886:1495143-1495382 [Pseudomonas frederiksbergensis]
ATGTCCGTCATGTCATTGCGTCTGCCAGACGAAATCGCCGACACCCTCGCCAATCTGGCTAAAGCGACAGGCCGCAGCAA
ATCTTTTCTCGCCGTAGATGCTCTACGTGAATACCTGGCTCGAGAAGCCTGGCAAATAGAGGAAATTCAACACGCCTTGA
AAGAGGCTGACGCTGGAGACTTTGCCAGTGATGAAGAAGTAAACGCCCTCGCCAACAAGTGGGCCAGCAATGCGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1J0EI29