Detailed information of TA system TA068256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1484501..1485122
• Replicon: chromosome
• Accession: NZ_CP017886
• Organism: Pseudomonas frederiksbergensis strain ERDD5:01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BLL42_RS07410
• Protein ID: WP_071551462.1
• Coordinates: 1484501..1484683 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1J0EI94
• Locus tag: BLL42_RS07415
• Protein ID: WP_071551463.1
• Coordinates: 1484715..1485122 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLL42_RS07385	1480393..1480599	+	207	WP_071551459.1	cysteine-rich CWC family protein	-
BLL42_RS07390	1480644..1481336	+	693	WP_071551460.1	pseudouridine synthase	-
BLL42_RS07395	1481648..1482532	+	885	WP_071551461.1	alpha/beta hydrolase	-
BLL42_RS07405	1483006..1484031	+	1026	WP_071550690.1	IS110 family transposase	-
BLL42_RS07410	1484501..1484683	+	183	WP_071551462.1	type II toxin-antitoxin system HicA family toxin	Toxin
BLL42_RS07415	1484715..1485122	+	408	WP_071551463.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BLL42_RS07420	1485147..1485917	-	771	WP_071551464.1	trans-aconitate 2-methyltransferase	-
BLL42_RS07425	1486113..1486442	-	330	WP_071551465.1	YXWGXW repeat-containing protein	-
BLL42_RS07430	1486749..1488284	+	1536	WP_071551466.1	DUF1800 domain-containing protein	-
BLL42_RS07435	1488306..1489502	+	1197	WP_071551467.1	DUF1501 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1483006..1484031	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6856.04 Da
• Isoelectric Point: 10.7851

>T68256 WP_071551462.1 NZ_CP017886:1484501-1484683 [Pseudomonas frederiksbergensis]
VNSRYLITQIVADGWYLVRVRGSHHHFKHPTKPGLVNVPHPKKDLLTKTAVSILQQALLY

Nucleotide

• Length: 183 bp

>T68256 NZ_CP017886:1484501-1484683 [Pseudomonas frederiksbergensis]
GTGAATAGCCGATATTTGATAACCCAAATCGTCGCAGACGGCTGGTATCTGGTACGGGTCAGGGGCAGCCACCACCACTT
CAAGCATCCAACGAAGCCCGGTCTGGTCAACGTCCCACACCCGAAAAAGGATTTACTGACCAAGACTGCCGTCAGCATCT
TGCAACAGGCCCTGCTGTATTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14556.52 Da
• Isoelectric Point: 4.4564

>AT68256 WP_071551463.1 NZ_CP017886:1484715-1485122 [Pseudomonas frederiksbergensis]
MLYPIAISMGDNEHAWGVEVPDIPGCFSAGDDLDDAIAMAREAIEGHFEILAEDGSAIPPANKVTVHAANPQYAGVTWAL
VDIDVTKYLGKAQKLNITLPGYLLTRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT68256 NZ_CP017886:1484715-1485122 [Pseudomonas frederiksbergensis]
ATGCTTTACCCAATTGCGATTTCAATGGGCGATAACGAACACGCCTGGGGCGTGGAGGTGCCGGATATTCCGGGCTGTTT
CTCGGCCGGTGATGACCTGGACGATGCGATCGCCATGGCGCGCGAAGCCATCGAAGGCCACTTCGAGATACTCGCCGAAG
ACGGCTCGGCGATTCCGCCCGCCAACAAAGTCACCGTGCATGCGGCCAATCCGCAGTACGCCGGCGTCACCTGGGCATTG
GTGGACATTGATGTCACCAAGTACCTGGGCAAGGCGCAGAAACTCAACATCACCCTGCCCGGCTATCTGCTGACCCGTAT
CGACGAATACGTACTGCATCACCCGGAAGAGAAAAGCCGCTCCGGCTTTCTGGCCTCGGCGGCGTTGAAAGTGTTGCAAC
AAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1J0EI94