Detailed information of TA system TA068245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 18074..18975
• Replicon: plasmid pCCDM105S
• Accession: NZ_CP017867
• Organism: Campylobacter coli strain YF2105

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: A0A3Z8TY88
• Locus tag: BLD35_RS09290
• Protein ID: WP_002814355.1
• Coordinates: 18074..18736 (-)
• Length: 221 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: C5F2H5
• Locus tag: BLD35_RS09295
• Protein ID: WP_002814351.1
• Coordinates: 18736..18975 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLD35_RS09255	13394..13777	+	384	WP_004288751.1	DNA transfer protein	-
BLD35_RS09260	13740..15896	+	2157	WP_057990995.1	relaxase/mobilization nuclease domain-containing protein	-
BLD35_RS09270	16137..16361	-	225	WP_002790621.1	hypothetical protein	-
BLD35_RS09275	16364..17050	-	687	WP_002791558.1	AAA family ATPase	-
BLD35_RS09280	17142..17537	-	396	WP_002782959.1	conjugal transfer protein TraM	-
BLD35_RS09285	17602..18069	-	468	WP_002782960.1	signal peptidase I	-
BLD35_RS09290	18074..18736	-	663	WP_002814355.1	Fic family protein	Toxin
BLD35_RS09295	18736..18975	-	240	WP_002814351.1	hypothetical protein	Antitoxin
BLD35_RS09300	19078..19797	-	720	WP_002844665.1	conjugal transfer protein TraL	-
BLD35_RS09305	19808..21631	-	1824	WP_057031751.1	type IV secretory system conjugative DNA transfer family protein	-
BLD35_RS09310	21628..23841	-	2214	WP_002790336.1	DUF1738 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25284	25284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (53-162)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 221 a.a.
• Molecular weight: 25478.46 Da
• Isoelectric Point: 10.1005

>T68245 WP_002814355.1 NZ_CP017867:c18736-18074 [Campylobacter coli]
MWDSQENLSEFDKFLIKTNKIGAKSLDELFRKERMITNKKALELGKNPIKGNFDYQHLKDIHKALFEDVYTWAGQDRMQM
GLKEKFAKYAPNGAIINFVPGKELDKYSKIIFDELAKNNYLKNSKDLNHFAKNLAKFMGEINALHPFREGNGRTQRIFLN
ELAKNAGYKLDLNLISKHKMIHACVEASQLKPGRLEALIKDNLKNFRQNLDLEQNKGMSL

Nucleotide

• Length: 663 bp

>T68245 NZ_CP017867:c18736-18074 [Campylobacter coli]
ATGTGGGATTCTCAAGAAAATTTATCAGAGTTTGATAAATTTTTAATCAAGACTAATAAAATAGGTGCAAAATCATTAGA
TGAATTGTTTCGCAAAGAAAGAATGATAACAAACAAAAAAGCCTTAGAATTAGGCAAAAATCCCATTAAAGGCAATTTTG
ATTATCAGCATTTAAAAGATATACATAAAGCACTTTTTGAAGATGTTTATACTTGGGCGGGACAAGATAGAATGCAAATG
GGGCTAAAAGAAAAATTTGCAAAATATGCACCCAATGGAGCAATTATTAATTTTGTTCCTGGAAAAGAGTTAGATAAATA
CTCTAAAATAATTTTTGATGAACTTGCAAAAAATAATTATCTAAAAAACTCCAAAGATCTAAATCATTTTGCAAAAAATT
TAGCAAAATTTATGGGCGAAATCAATGCTTTACACCCTTTTAGAGAAGGTAACGGCAGAACTCAAAGAATATTTTTAAAT
GAATTAGCTAAAAATGCAGGTTATAAACTTGATTTAAATTTAATTTCTAAGCATAAAATGATACATGCTTGCGTAGAAGC
TTCACAATTAAAACCTGGAAGATTGGAGGCTTTAATTAAAGACAATCTAAAAAACTTTAGACAAAATTTAGATTTGGAGC
AAAATAAAGGAATGTCGTTATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9089.40 Da
• Isoelectric Point: 4.6535

>AT68245 WP_002814351.1 NZ_CP017867:c18975-18736 [Campylobacter coli]
MQKQYPEVHSLEESLAILKKYKDDLTKEQYEAIRSNIGNFAIEDMFLNEKDIIDNVKIIKGEATANECIVALKKEWGVS

Nucleotide

• Length: 240 bp

>AT68245 NZ_CP017867:c18975-18736 [Campylobacter coli]
ATGCAAAAACAATATCCCGAAGTGCATAGCTTAGAAGAAAGCCTTGCGATACTTAAAAAATATAAAGATGATTTGACTAA
AGAGCAATACGAAGCCATAAGATCAAATATAGGTAATTTTGCAATAGAAGATATGTTTTTGAATGAAAAAGATATTATTG
ATAATGTTAAAATTATAAAAGGCGAAGCAACAGCTAATGAATGTATTGTTGCATTAAAGAAAGAATGGGGAGTGTCTTAA

Structures

Toxin

• AlphaFold DB: A0A3Z8TY88

Antitoxin

• AlphaFold DB: A0A3Z9DV79