Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4751293..4751950 Replicon chromosome
Accession NZ_CP017849
Organism Klebsiella variicola strain GJ2

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag BBD64_RS23405 Protein ID WP_002916310.1
Coordinates 4751293..4751703 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag BBD64_RS23410 Protein ID WP_002916312.1
Coordinates 4751684..4751950 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD64_RS23385 4747293..4749026 - 1734 WP_064166671.1 single-stranded-DNA-specific exonuclease RecJ -
BBD64_RS23390 4749032..4749745 - 714 WP_077139430.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BBD64_RS23395 4749768..4750664 - 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
BBD64_RS23400 4750765..4751286 + 522 WP_008806427.1 flavodoxin FldB -
BBD64_RS23405 4751293..4751703 - 411 WP_002916310.1 protein YgfX Toxin
BBD64_RS23410 4751684..4751950 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
BBD64_RS23415 4752196..4753179 + 984 WP_032733797.1 tRNA-modifying protein YgfZ -
BBD64_RS23420 4753387..4754046 - 660 WP_008806429.1 hemolysin III family protein -
BBD64_RS23425 4754210..4754521 - 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
BBD64_RS23430 4754572..4755300 + 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
BBD64_RS23435 4755420..4756853 + 1434 WP_063105732.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T68236 WP_002916310.1 NZ_CP017849:c4751703-4751293 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T68236 NZ_CP017849:c4751703-4751293 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT68236 WP_002916312.1 NZ_CP017849:c4751950-4751684 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT68236 NZ_CP017849:c4751950-4751684 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References