Detailed information of TA system TA068235

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4344480..4345263
• Replicon: chromosome
• Accession: NZ_CP017849
• Organism: Klebsiella variicola strain GJ2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BBD64_RS21325
• Protein ID: WP_167353669.1
• Coordinates: 4344895..4345263 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BBD64_RS21320
• Protein ID: WP_040018969.1
• Coordinates: 4344480..4344839 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD64_RS21275	4339697..4340554	+	858	WP_032705139.1	hypothetical protein	-
BBD64_RS21280	4340832..4341296	+	465	WP_004114621.1	hypothetical protein	-
BBD64_RS21285	4341351..4341710	+	360	WP_004114622.1	hypothetical protein	-
BBD64_RS21290	4341796..4342008	+	213	WP_004114623.1	hypothetical protein	-
BBD64_RS21295	4342119..4342940	+	822	WP_040018972.1	DUF945 domain-containing protein	-
BBD64_RS21300	4343020..4343499	+	480	WP_040018971.1	antirestriction protein	-
BBD64_RS21310	4343740..4344222	+	483	WP_004114630.1	DNA repair protein RadC	-
BBD64_RS21315	4344236..4344457	+	222	WP_004114632.1	DUF987 domain-containing protein	-
BBD64_RS21320	4344480..4344839	+	360	WP_040018969.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BBD64_RS21325	4344895..4345263	+	369	WP_167353669.1	TA system toxin CbtA family protein	Toxin
BBD64_RS21330	4345343..4345663	-	321	WP_004114640.1	hypothetical protein	-
BBD64_RS21335	4345802..4346791	-	990	WP_004114642.1	glycosyltransferase	-
BBD64_RS21345	4347414..4348331	-	918	WP_004114644.1	hypothetical protein	-
BBD64_RS21350	4348418..4349320	+	903	WP_077139515.1	bacteriophage abortive infection AbiH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4320224..4362575	42351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (7-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.52 Da
• Isoelectric Point: 9.4643

>T68235 WP_167353669.1 NZ_CP017849:4344895-4345263 [Klebsiella variicola]
IQSLPPQRAASSRLSSVEIWQKLLEYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGFSV
KEQSPFIGSLDMLRARKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 369 bp

>T68235 NZ_CP017849:4344895-4345263 [Klebsiella variicola]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACGACACGCCGTTTGGCAACGACAGTGTGATCCAGAAACACATTGATGCGG
GAATCTCACTGTGTGACGCTGTAAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGCCCGGAAGGCTACCGGGCTGATGACCCGCAAGGGATA
TAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13349.56 Da
• Isoelectric Point: 8.1516

>AT68235 WP_040018969.1 NZ_CP017849:4344480-4344839 [Klebsiella variicola]
MKKATRIINHNIAEPWWGLRRNITPCLGARLVQEGNRLHYLADRASIAGTFNDTDLRHLDQAFPVLMKQMELMLASSELT
PHIQRCITLHAKGLICEADTLGSCGYLYIVIYPTSAITA

Nucleotide

• Length: 360 bp

>AT68235 NZ_CP017849:4344480-4344839 [Klebsiella variicola]
ATGAAGAAAGCAACCCGTATAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACA
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAGCAGCGAACTCACG
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCTGATACGCTCGGCTCCTGTGGCTATTT
GTATATCGTAATTTATCCGACATCTGCAATAACCGCATAA