Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3720735..3721325 Replicon chromosome
Accession NZ_CP017849
Organism Klebsiella variicola strain GJ2

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag BBD64_RS18430 Protein ID WP_008804165.1
Coordinates 3720735..3721067 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag BBD64_RS18435 Protein ID WP_012541132.1
Coordinates 3721068..3721325 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD64_RS18405 3716773..3717018 + 246 WP_008804171.1 signal transduction protein PmrD -
BBD64_RS18410 3717149..3718603 + 1455 WP_008804170.1 AMP nucleosidase -
BBD64_RS18420 3718976..3720412 - 1437 WP_162494461.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
BBD64_RS18430 3720735..3721067 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BBD64_RS18435 3721068..3721325 - 258 WP_012541132.1 antitoxin Antitoxin
BBD64_RS18440 3721854..3722954 + 1101 WP_048268588.1 phosphotransferase -
BBD64_RS18445 3722951..3723922 - 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
BBD64_RS18450 3724128..3724964 - 837 WP_008804155.1 alpha/beta hydrolase -
BBD64_RS18455 3725141..3725716 - 576 WP_077139353.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T68234 WP_008804165.1 NZ_CP017849:c3721067-3720735 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T68234 NZ_CP017849:c3721067-3720735 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT68234 WP_012541132.1 NZ_CP017849:c3721325-3721068 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT68234 NZ_CP017849:c3721325-3721068 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References