Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1263097..1263716 Replicon chromosome
Accession NZ_CP017849
Organism Klebsiella variicola strain GJ2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BBD64_RS06195 Protein ID WP_002892050.1
Coordinates 1263097..1263315 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag BBD64_RS06200 Protein ID WP_008805436.1
Coordinates 1263342..1263716 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD64_RS06165 1259140..1259403 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
BBD64_RS06170 1259403..1259543 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BBD64_RS06175 1259540..1260238 - 699 WP_012968815.1 GNAT family N-acetyltransferase -
BBD64_RS06180 1260339..1261796 + 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
BBD64_RS06185 1261765..1262235 - 471 WP_008805434.1 YlaC family protein -
BBD64_RS30575 1262272..1262400 + 129 Protein_1139 hypothetical protein -
BBD64_RS06190 1262372..1262938 - 567 WP_032438710.1 maltose O-acetyltransferase -
BBD64_RS06195 1263097..1263315 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BBD64_RS06200 1263342..1263716 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
BBD64_RS06210 1264199..1267345 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
BBD64_RS06215 1267368..1268561 - 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T68223 WP_002892050.1 NZ_CP017849:c1263315-1263097 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T68223 NZ_CP017849:c1263315-1263097 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT68223 WP_008805436.1 NZ_CP017849:c1263716-1263342 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT68223 NZ_CP017849:c1263716-1263342 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References