Detailed information of TA system TA068213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3990..4515
• Replicon: plasmid pEcoFMU07332d
• Accession: NZ_CP017848
• Organism: Escherichia coli strain FMU073332

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: BLJ80_RS26900
• Protein ID: WP_001159871.1
• Coordinates: 3990..4295 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: BLJ80_RS26905
• Protein ID: WP_072328065.1
• Coordinates: 4297..4515 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLJ80_RS29620	1..273	+	273	Protein_0	IS110 family transposase	-
BLJ80_RS29875	526..1756	-	1231	Protein_1	IS66 family transposase	-
BLJ80_RS26885	1966..2274	-	309	WP_000030204.1	molecular chaperone GroEL	-
BLJ80_RS26890	2361..3005	-	645	WP_001144037.1	ParA family protein	-
BLJ80_RS26895	3183..3989	-	807	WP_000016960.1	site-specific integrase	-
BLJ80_RS26900	3990..4295	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
BLJ80_RS26905	4297..4515	-	219	WP_072328065.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BLJ80_RS26920	5037..5294	+	258	WP_000343095.1	hypothetical protein	-
BLJ80_RS26925	5294..5836	+	543	WP_032328642.1	hypothetical protein	-
BLJ80_RS26930	6080..6922	+	843	WP_160371899.1	polysaccharide deacetylase family protein	-
BLJ80_RS26935	6925..8013	+	1089	WP_064734538.1	putative hexosyltransferase CapU	-
BLJ80_RS26940	8018..8968	+	951	WP_032328199.1	virulence factor VirK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltB / eltA / etpA / etpB / cs3	1..137665	137665

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T68213 WP_001159871.1 NZ_CP017848:c4295-3990 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T68213 NZ_CP017848:c4295-3990 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8470.54 Da
• Isoelectric Point: 6.3212

>AT68213 WP_072328065.1 NZ_CP017848:c4515-4297 [Escherichia coli]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVTRFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT68213 NZ_CP017848:c4515-4297 [Escherichia coli]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCACCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8