Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 10247..11045 Replicon plasmid pEcoFMU073332c
Accession NZ_CP017847
Organism Escherichia coli strain FMU073332

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag BLJ80_RS26280 Protein ID WP_000072677.1
Coordinates 10524..11045 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag BLJ80_RS26275 Protein ID WP_001351987.1
Coordinates 10247..10516 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLJ80_RS26245 5520..6542 + 1023 WP_001295213.1 IS21 family transposase -
BLJ80_RS26250 6539..7321 + 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
BLJ80_RS26255 7388..7798 - 411 Protein_8 hypothetical protein -
BLJ80_RS26260 8081..8848 + 768 WP_000342417.1 hypothetical protein -
BLJ80_RS26265 8901..9254 - 354 WP_160378290.1 hypothetical protein -
BLJ80_RS26270 9260..9928 - 669 WP_000161228.1 AAA family ATPase -
BLJ80_RS26275 10247..10516 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
BLJ80_RS26280 10524..11045 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
BLJ80_RS26290 11214..11465 - 252 WP_000901559.1 hypothetical protein -
BLJ80_RS26295 11467..12159 - 693 WP_012640731.1 hypothetical protein -
BLJ80_RS26300 12173..12496 - 324 WP_001717323.1 hypothetical protein -
BLJ80_RS28940 12699..15365 - 2667 WP_077891209.1 tail fiber protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..113343 113343


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T68212 WP_000072677.1 NZ_CP017847:10524-11045 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T68212 NZ_CP017847:10524-11045 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTCACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT68212 WP_001351987.1 NZ_CP017847:10247-10516 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT68212 NZ_CP017847:10247-10516 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References