Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3731695..3732333 Replicon chromosome
Accession NZ_CP017844
Organism Escherichia coli strain FMU073332

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BLJ80_RS20210 Protein ID WP_000813794.1
Coordinates 3732157..3732333 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BLJ80_RS20205 Protein ID WP_001270286.1
Coordinates 3731695..3732111 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLJ80_RS20185 3726847..3727788 - 942 WP_001251313.1 ABC transporter permease -
BLJ80_RS20190 3727789..3728802 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
BLJ80_RS20195 3728820..3729965 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
BLJ80_RS20200 3730210..3731616 - 1407 WP_000760586.1 PLP-dependent aminotransferase family protein -
BLJ80_RS20205 3731695..3732111 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BLJ80_RS20210 3732157..3732333 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BLJ80_RS20215 3732555..3732785 + 231 WP_000494244.1 YncJ family protein -
BLJ80_RS20220 3732877..3734838 - 1962 WP_001355676.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BLJ80_RS20225 3734911..3735447 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
BLJ80_RS20230 3735500..3736714 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T68211 WP_000813794.1 NZ_CP017844:c3732333-3732157 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T68211 NZ_CP017844:c3732333-3732157 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT68211 WP_001270286.1 NZ_CP017844:c3732111-3731695 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT68211 NZ_CP017844:c3732111-3731695 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References