Detailed information of TA system TA068181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 2379011..2379534
• Replicon: chromosome
• Accession: NZ_CP017810
• Organism: Thalassolituus oleivorans strain K188

Toxin (Protein)

• Gene name: parE
• Uniprot ID: W8G9R1
• Locus tag: CN03_RS10990
• Protein ID: WP_025265720.1
• Coordinates: 2379011..2379295 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: W8G5L0
• Locus tag: CN03_RS10995
• Protein ID: WP_025265721.1
• Coordinates: 2379292..2379534 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CN03_RS10970	2376341..2377135	+	795	WP_025265716.1	uroporphyrinogen-III C-methyltransferase	-
CN03_RS10975	2377809..2378225	+	417	WP_025265717.1	GNAT family N-acetyltransferase	-
CN03_RS10980	2378347..2378610	+	264	WP_025265718.1	ribbon-helix-helix protein, CopG family	-
CN03_RS10985	2378607..2378897	+	291	WP_051482463.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CN03_RS10990	2379011..2379295	-	285	WP_025265720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CN03_RS10995	2379292..2379534	-	243	WP_025265721.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CN03_RS11000	2379623..2379874	-	252	WP_025265722.1	hypothetical protein	-
CN03_RS11005	2380096..2380323	+	228	WP_081758370.1	hypothetical protein	-
CN03_RS11010	2380383..2380760	+	378	WP_025265724.1	hypothetical protein	-
CN03_RS11015	2380757..2380990	+	234	WP_025265725.1	hypothetical protein	-
CN03_RS11020	2381271..2381828	+	558	WP_075273803.1	hypothetical protein	-
CN03_RS11025	2381936..2382568	-	633	WP_083615678.1	sulfotransferase family protein	-
CN03_RS11030	2382671..2383018	-	348	WP_025265728.1	hypothetical protein	-
CN03_RS11035	2383097..2383669	-	573	WP_015486594.1	DNA-3-methyladenine glycosylase I	-
CN03_RS11040	2383739..2384389	-	651	WP_075273805.1	DUF938 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10806.57 Da
• Isoelectric Point: 9.7129

>T68181 WP_025265720.1 NZ_CP017810:c2379295-2379011 [Thalassolituus oleivorans]
MTSFTLTTRAKSDLLAIAKFTQVRWGLAQRNIYIKQFDDAFHMLAASPTLGKNCDYIKDGYYKFPQGSHIIFYKFTADGP
LQIIRILHEKMDVA

Nucleotide

• Length: 285 bp

>T68181 NZ_CP017810:c2379295-2379011 [Thalassolituus oleivorans]
ATGACTTCATTTACGCTAACCACTCGCGCCAAAAGTGATTTGTTAGCTATTGCCAAATTTACGCAAGTTCGCTGGGGGCT
GGCGCAGCGTAATATTTATATCAAGCAGTTTGATGATGCCTTTCATATGCTAGCGGCGTCACCCACCTTAGGAAAAAACT
GTGATTATATTAAAGACGGATATTATAAATTCCCTCAAGGCAGTCACATAATATTCTATAAATTTACAGCAGATGGACCT
TTACAGATTATTCGAATACTTCATGAAAAAATGGATGTAGCTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8924.86 Da
• Isoelectric Point: 4.5023

>AT68181 WP_025265721.1 NZ_CP017810:c2379534-2379292 [Thalassolituus oleivorans]
MAKNTSMTLGEHFDVFIAHQIESGRYSSASEVIRAGLRALEDHESKLEVLRQMLKDGEASGVAEYSYESFVAELDNENSK

Nucleotide

• Length: 243 bp

>AT68181 NZ_CP017810:c2379534-2379292 [Thalassolituus oleivorans]
ATGGCTAAAAACACTAGTATGACACTCGGTGAGCACTTTGATGTTTTTATAGCTCATCAGATTGAAAGCGGCCGCTACAG
CTCTGCAAGTGAGGTAATACGCGCAGGTCTGAGGGCACTTGAAGACCACGAAAGTAAGCTCGAAGTATTGCGGCAGATGC
TTAAAGACGGAGAGGCTAGTGGCGTTGCTGAGTATAGTTATGAGTCTTTCGTAGCTGAGCTTGATAATGAAAACAGCAAA
TGA

Structures

Toxin

• AlphaFold DB: W8G9R1

Antitoxin

• AlphaFold DB: W8G5L0