Detailed information of TA system TA068180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1763667..1765357
• Replicon: chromosome
• Accession: NZ_CP017810
• Organism: Thalassolituus oleivorans strain K188

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: A0A1L6KCV6
• Locus tag: CN03_RS08115
• Protein ID: WP_075273277.1
• Coordinates: 1764038..1765357 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: CN03_RS08110
• Protein ID: WP_025265361.1
• Coordinates: 1763667..1764041 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CN03_RS08090	1760216..1760638	+	423	WP_051052472.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
CN03_RS08095	1760672..1761514	+	843	WP_015487177.1	zinc-dependent peptidase	-
CN03_RS08100	1761579..1762940	-	1362	WP_075273276.1	DEAD/DEAH box helicase	-
CN03_RS08105	1762950..1763498	-	549	WP_015487175.1	YaeQ family protein	-
CN03_RS08110	1763667..1764041	+	375	WP_025265361.1	helix-turn-helix domain-containing protein	Antitoxin
CN03_RS08115	1764038..1765357	+	1320	WP_075273277.1	type II toxin-antitoxin system HipA family toxin	Toxin
CN03_RS08120	1765428..1765892	-	465	WP_075273278.1	hypothetical protein	-
CN03_RS08125	1765933..1767876	-	1944	WP_075273279.1	EAL domain-containing protein	-
CN03_RS08130	1768071..1768235	-	165	WP_083615651.1	DUF2789 family protein	-
CN03_RS08135	1768272..1769375	-	1104	WP_083615652.1	acyl-CoA desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (175-415)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49174.04 Da
• Isoelectric Point: 6.7690

>T68180 WP_075273277.1 NZ_CP017810:1764038-1765357 [Thalassolituus oleivorans]
VISVAEVRLWGITIGAVQWDAQRQLGFFEYAKPFLRSGIQLSPLHMPLGAGVFSFPALNKESYKGLPGMLADSLPDKFGN
LLIDQWLVSQNREPGSFNPVERLLYMGIRGMGALEYLPETPRTRIPDQQLEMTKLVDLANRALAQKDSLAAKFGYSANDS
MADTQALQQIISVGTSAGGARAKAVIAWNEATHEVRSGQLDCPEGFEHYLLKFDGVQGNRDKELNDPQGFTRIEYAYSQM
AKAAGIDMMPCQLLEENGRAHFLTKRFDRIGGKDKLHMQTLCGVAHFDFNMAGAYSYEQAFRVMRQMGLQDLHLALEQQF
RRMVFNVMARNQDDHTKNIAFLMNKRGEWRLSPAYDVTYSYNPSGEWTSSHQMSINGKRDGFVLDDLLACGAMADVSERR
VRAIVGEVAEVLKTWSQYAEDAGIEVSWATKIGASFRKI

Nucleotide

• Length: 1320 bp

>T68180 NZ_CP017810:1764038-1765357 [Thalassolituus oleivorans]
GTGATTTCTGTGGCAGAAGTGCGTCTCTGGGGAATAACCATTGGTGCTGTGCAATGGGATGCGCAGCGTCAGCTTGGTTT
CTTTGAATACGCTAAGCCTTTTTTACGCTCGGGCATTCAGCTTTCGCCATTGCATATGCCGCTGGGTGCCGGCGTGTTTT
CATTTCCTGCTTTGAATAAAGAATCGTACAAGGGTTTGCCAGGTATGTTGGCGGACTCTTTGCCTGATAAATTCGGTAAT
TTACTGATAGATCAGTGGTTAGTGAGTCAGAATCGTGAACCGGGCAGTTTTAACCCCGTGGAACGTCTTTTGTATATGGG
CATACGTGGTATGGGCGCGCTTGAGTATTTACCAGAAACTCCACGCACTCGCATTCCTGATCAGCAATTAGAAATGACTA
AACTGGTGGATTTGGCTAATCGAGCCCTAGCTCAAAAGGATAGCTTGGCTGCTAAGTTTGGTTATTCTGCCAATGACTCT
ATGGCCGATACTCAAGCCTTGCAGCAGATTATTTCTGTGGGTACGTCGGCTGGTGGTGCTCGCGCTAAAGCCGTGATTGC
ATGGAATGAGGCAACTCATGAAGTTCGCTCTGGGCAATTGGATTGTCCTGAGGGTTTTGAGCATTATTTGTTGAAGTTCG
ATGGTGTGCAGGGTAACCGTGATAAAGAGTTGAATGATCCACAAGGATTTACTCGTATCGAATATGCCTACTCGCAAATG
GCTAAAGCCGCTGGGATCGACATGATGCCGTGTCAGTTGTTAGAAGAAAATGGGCGTGCGCACTTTCTAACAAAACGTTT
TGATCGAATAGGCGGCAAAGATAAACTGCACATGCAAACGCTCTGTGGTGTTGCGCACTTCGATTTTAATATGGCGGGTG
CGTATTCTTATGAGCAAGCGTTTCGGGTGATGCGCCAAATGGGCTTGCAGGATTTGCATCTAGCACTTGAGCAGCAATTT
CGTCGTATGGTGTTTAACGTTATGGCACGTAATCAGGACGACCATACGAAAAATATCGCTTTCTTAATGAATAAGCGCGG
TGAATGGCGCTTGTCTCCCGCCTATGATGTGACCTATTCGTATAATCCTAGCGGCGAGTGGACGTCGAGTCATCAAATGA
GTATTAACGGCAAACGCGATGGATTTGTACTGGATGATCTGCTTGCTTGTGGCGCGATGGCGGATGTCAGTGAGCGCCGT
GTTAGAGCGATAGTGGGTGAAGTGGCGGAGGTATTAAAAACTTGGTCTCAATATGCTGAGGATGCGGGTATTGAGGTAAG
TTGGGCGACTAAGATTGGCGCCAGCTTCCGTAAAATATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13336.95 Da
• Isoelectric Point: 4.6151

>AT68180 WP_025265361.1 NZ_CP017810:1763667-1764041 [Thalassolituus oleivorans]
MEYSSVSNLVVLEQLGERFRSARLSRNLTQAQLAEASGVSLSTLRRLESGADNVSLMNIIALMRALGVVDQLELLLPPQP
PSPMASLQNSGAGPQVDNGSERQRASGSRQVAEEKGTWTWGDDA

Nucleotide

• Length: 375 bp

>AT68180 NZ_CP017810:1763667-1764041 [Thalassolituus oleivorans]
ATGGAATACTCATCAGTTAGTAATTTAGTCGTGCTAGAGCAGTTGGGAGAGCGTTTTCGCAGTGCTCGTTTAAGCCGTAA
CTTAACTCAAGCGCAGTTGGCAGAGGCATCGGGCGTCAGCTTGTCGACGCTAAGGCGTTTGGAGAGCGGGGCGGATAACG
TCAGCTTAATGAATATTATCGCCTTAATGCGCGCCCTAGGTGTGGTCGATCAACTTGAATTGTTACTACCGCCTCAGCCT
CCTAGTCCTATGGCGAGCTTGCAGAATAGCGGTGCAGGGCCGCAAGTCGATAACGGCAGCGAGCGGCAGCGTGCATCTGG
CAGCCGTCAGGTTGCCGAAGAAAAGGGCACTTGGACATGGGGCGATGACGCGTGA

Structures

Toxin

• AlphaFold DB: A0A1L6KCV6