Detailed information of TA system TA068167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2889978..2891581
• Replicon: chromosome
• Accession: NZ_CP017802
• Organism: Raoultella ornithinolytica strain MG isolate MG01

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BK817_RS13665
• Protein ID: WP_064367563.1
• Coordinates: 2890250..2891581 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: BK817_RS13660
• Protein ID: WP_032717448.1
• Coordinates: 2889978..2890250 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK817_RS13635	2885596..2886837	+	1242	WP_071359402.1	sensor domain-containing diguanylate cyclase	-
BK817_RS13640	2886857..2887489	+	633	WP_048023555.1	class I SAM-dependent methyltransferase	-
BK817_RS13645	2887703..2888656	+	954	WP_004861921.1	hypothetical protein	-
BK817_RS13650	2888790..2889215	+	426	WP_032724724.1	hypothetical protein	-
BK817_RS13655	2889221..2889775	+	555	WP_004861926.1	RNA polymerase sigma factor	-
BK817_RS13660	2889978..2890250	+	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
BK817_RS13665	2890250..2891581	+	1332	WP_064367563.1	type II toxin-antitoxin system HipA family toxin	Toxin
BK817_RS13670	2891514..2892929	-	1416	WP_099144431.1	glycoside hydrolase family 10 protein	-
BK817_RS13675	2893635..2894693	+	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
BK817_RS13680	2894793..2895707	+	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2765148..2932916	167768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49313.63 Da
• Isoelectric Point: 6.7010

>T68167 WP_064367563.1 NZ_CP017802:2890250-2891581 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGDQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T68167 NZ_CP017802:2890250-2891581 [Raoultella ornithinolytica]
ATGGCGACTCTCGTGACATGGATGAACAATGAGCGGGTCGGCGAGCTAATCAAGCAGGCGAACGGCGCACATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCTCGCGCCAGACCGCTCTCACTGTCGCTTCCGCTGCAGTACGGTAACATCACCG
ACGAGGCGGTATTTAACTACTTTGACAATCTGCTGCCGGACAGTCCGCGGGTACGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCGTTTGATCTGCTGGCCGAGATCGGACGGGACAGCGTCGGGGCGGTAACGTTACTCCCGCCAGG
AGAAAATGCGCCCCGCGGGGCGTTGAGCTGGGAGCCGCTGGATAACCACGCTCTGGATGCGTTACTGACGGCCTACCAGT
CGGATATACCGCTCGGAATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGATCAGTGGTGTATCCCGACGGGTACCACGCCGACTACCCACATCATTAAACTGCCCATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTCGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCCCGCCAGCATTATCCAGACAGCCAGTATCAGGGCGCTGGCGGTGGAGCGTTTCGACCGTCGCTGG
GCGCAGGAGAAAACGGTGCTGCTGCGTTTGCCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCAATTATGGCTTTTCTGGTTGGCTCCAGCGAGGCACTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCCAAAAACTTCTCGATATTCCTCCAG
CCGGGCGGCAGCTACCGCTTAACCCCGTTCTACGACATTATCTCCGCGTTTCCGCTGCTTGGCGGAAAGGGACTGCACCT
GAGCGATCTCAAGCTGTCGATGAGTCTGAAGGCCAGTAAAGGACGTAAAACGGAGATCCAGACGCTCTATCCGCGTCACT
TTCTCGCTACGGCTAAAGAGGTAGGGTTTGCCCAGCAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CGCGCGGTTGAGGCGGTAAAGGGGACTTTACCGCCTGACTTTTCACAACCGGTATATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTCTCCGTCGGGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT68167 WP_032717448.1 NZ_CP017802:2889978-2890250 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT68167 NZ_CP017802:2889978-2890250 [Raoultella ornithinolytica]
ATGAGCCCTTCACCAACGATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGAGCTGGCTCAGCGTATGGGGATTAAACAGGCCACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCGACGAT
AACCGTGATAACCCGCGGGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5