Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1450410..1451029 Replicon chromosome
Accession NZ_CP017802
Organism Raoultella ornithinolytica strain MG isolate MG01

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag BK817_RS06895 Protein ID WP_004858783.1
Coordinates 1450410..1450628 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag BK817_RS06900 Protein ID WP_004858785.1
Coordinates 1450655..1451029 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK817_RS06865 1446483..1446743 + 261 WP_004858777.1 type B 50S ribosomal protein L31 -
BK817_RS06870 1446746..1446886 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BK817_RS06875 1446883..1447593 - 711 WP_015585015.1 GNAT family N-acetyltransferase -
BK817_RS06880 1447696..1449147 + 1452 WP_071359190.1 PLP-dependent aminotransferase family protein -
BK817_RS06885 1449122..1449592 - 471 WP_004858780.1 YlaC family protein -
BK817_RS06890 1449692..1450258 - 567 WP_004858781.1 maltose O-acetyltransferase -
BK817_RS06895 1450410..1450628 - 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
BK817_RS06900 1450655..1451029 - 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
BK817_RS06905 1451557..1454703 - 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit -
BK817_RS06910 1454726..1455919 - 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T68165 WP_004858783.1 NZ_CP017802:c1450628-1450410 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T68165 NZ_CP017802:c1450628-1450410 [Raoultella ornithinolytica]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT68165 WP_004858785.1 NZ_CP017802:c1451029-1450655 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT68165 NZ_CP017802:c1451029-1450655 [Raoultella ornithinolytica]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGATCCAACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGTCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References