Detailed information of TA system TA068164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 880754..881333
• Replicon: chromosome
• Accession: NZ_CP017802
• Organism: Raoultella ornithinolytica strain MG isolate MG01

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: BK817_RS04170
• Protein ID: WP_032716505.1
• Coordinates: 880754..881041 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: BK817_RS04175
• Protein ID: WP_071359132.1
• Coordinates: 881028..881333 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK817_RS04140	876249..876767	-	519	WP_015585229.1	hypothetical protein	-
BK817_RS04145	876764..877576	-	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
BK817_RS04150	877720..878298	-	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
BK817_RS04155	878446..879228	-	783	WP_071359130.1	NAD(P)H-dependent oxidoreductase	-
BK817_RS04160	879316..880224	+	909	WP_004857513.1	LysR family transcriptional regulator	-
BK817_RS04165	880218..880637	+	420	WP_071359131.1	FosA family fosfomycin resistance glutathione transferase	-
BK817_RS04170	880754..881041	+	288	WP_032716505.1	BrnT family toxin	Toxin
BK817_RS04175	881028..881333	+	306	WP_071359132.1	BrnA antitoxin family protein	Antitoxin
BK817_RS04180	881488..882900	-	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
BK817_RS04185	883078..883242	+	165	WP_004857521.1	DUF1127 domain-containing protein	-
BK817_RS04190	883449..883913	+	465	WP_004857523.1	MarR family transcriptional regulator	-
BK817_RS04195	883920..884987	+	1068	WP_004857525.1	HlyD family secretion protein	-
BK817_RS04200	884977..886041	+	1065	WP_004857527.1	DUF2955 domain-containing protein	-
BK817_RS04205	886038..886292	-	255	WP_004857528.1	DUF3343 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T68164 WP_032716505.1 NZ_CP017802:880754-881041 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T68164 NZ_CP017802:880754-881041 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11681.22 Da
• Isoelectric Point: 9.4429

>AT68164 WP_071359132.1 NZ_CP017802:881028-881333 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKKS

Nucleotide

• Length: 306 bp

>AT68164 NZ_CP017802:881028-881333 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAACGCGATTCTGCGGGAAGCCATGCTGCGCGAGCAGAATAAGAAATCGTAA

Structures

Toxin

• AlphaFold DB: A0A808YBH6