Detailed information of TA system TA068162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 49189..49796
• Replicon: plasmid unnamed2
• Accession: NZ_CP017799
• Organism: Enterococcus faecium strain E243

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: BK696_RS16110
• Protein ID: WP_002321032.1
• Coordinates: 49446..49796 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: BK696_RS16105
• Protein ID: WP_002287514.1
• Coordinates: 49189..49452 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK696_RS16065	44539..45012	+	474	WP_002321302.1	single-stranded DNA-binding protein	-
BK696_RS16070	45378..45638	+	261	WP_002321303.1	hypothetical protein	-
BK696_RS16075	46083..46289	+	207	WP_002296239.1	hypothetical protein	-
BK696_RS16080	46289..46540	+	252	WP_002305766.1	hypothetical protein	-
BK696_RS16085	46556..46972	+	417	WP_002305764.1	hypothetical protein	-
BK696_RS16090	46954..47226	+	273	WP_002348912.1	hypothetical protein	-
BK696_RS16095	47393..47794	+	402	WP_002305759.1	IS200/IS605 family transposase	-
BK696_RS16100	47806..48951	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
BK696_RS16105	49189..49452	+	264	WP_002287514.1	hypothetical protein	Antitoxin
BK696_RS16110	49446..49796	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BK696_RS16115	49820..50434	-	615	WP_002318230.1	recombinase family protein	-
BK696_RS16120	50884..52209	+	1326	WP_002348911.1	Y-family DNA polymerase	-
BK696_RS16125	52202..52552	+	351	WP_002303114.1	hypothetical protein	-
BK696_RS16130	52549..52728	+	180	WP_002322386.1	hypothetical protein	-
BK696_RS16135	52740..53033	+	294	WP_002322251.1	hypothetical protein	-
BK696_RS16140	53259..54077	+	819	WP_002305212.1	ParA family protein	-
BK696_RS16145	54096..54503	+	408	WP_002305774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54822	54822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T68162 WP_002321032.1 NZ_CP017799:49446-49796 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T68162 NZ_CP017799:49446-49796 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT68162 WP_002287514.1 NZ_CP017799:49189-49452 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT68162 NZ_CP017799:49189-49452 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0