Detailed information of TA system TA068153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1096940..1097576
• Replicon: chromosome
• Accession: NZ_CP017786
• Organism: Bacillus xiamenensis strain VV3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K2MHT4
• Locus tag: BK049_RS05655
• Protein ID: WP_003214169.1
• Coordinates: 1096940..1097290 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K2P1A0
• Locus tag: BK049_RS05660
• Protein ID: WP_008359553.1
• Coordinates: 1097295..1097576 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK049_RS05615	1091970..1092569	-	600	WP_008359571.1	SpoIIE family protein phosphatase	-
BK049_RS05620	1092569..1093357	-	789	WP_008359569.1	RNA polymerase sigma factor SigB	-
BK049_RS05625	1093323..1093811	-	489	WP_008359566.1	anti-sigma B factor RsbW	-
BK049_RS05630	1093808..1094137	-	330	WP_008359565.1	anti-sigma factor antagonist	-
BK049_RS05635	1094197..1095204	-	1008	WP_008359562.1	PP2C family protein-serine/threonine phosphatase	-
BK049_RS05640	1095215..1095616	-	402	WP_008359561.1	anti-sigma regulatory factor	-
BK049_RS05645	1095619..1095987	-	369	WP_008359559.1	RsbT antagonist protein RsbS	-
BK049_RS05650	1095992..1096825	-	834	WP_174549250.1	RsbT co-antagonist protein RsbRA	-
BK049_RS05655	1096940..1097290	-	351	WP_003214169.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BK049_RS05660	1097295..1097576	-	282	WP_008359553.1	hypothetical protein	Antitoxin
BK049_RS05665	1097873..1099051	-	1179	WP_071168044.1	alanine racemase	-
BK049_RS05670	1099192..1100208	-	1017	WP_145939394.1	outer membrane lipoprotein-sorting protein	-
BK049_RS05675	1100369..1100734	-	366	WP_071168045.1	holo-ACP synthase	-
BK049_RS05680	1100829..1101434	+	606	WP_071168046.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.99 Da
• Isoelectric Point: 5.1663

>T68153 WP_003214169.1 NZ_CP017786:c1097290-1096940 [Bacillus xiamenensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T68153 NZ_CP017786:c1097290-1096940 [Bacillus xiamenensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATCCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTCTTGGAACAGATT
CGTACGATTGATAAACAAAGATTGACAGATAAAATTACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10836.19 Da
• Isoelectric Point: 4.9446

>AT68153 WP_008359553.1 NZ_CP017786:c1097576-1097295 [Bacillus xiamenensis]
MSESSATNEMKIQLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68153 NZ_CP017786:c1097576-1097295 [Bacillus xiamenensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCAACTGCCGGAGGCTTTAATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Structures

Toxin

• AlphaFold DB: K2MHT4

Antitoxin

• AlphaFold DB: K2P1A0