Detailed information of TA system TA068152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 104253..104989
• Replicon: plasmid unnamed2
• Accession: NZ_CP017779
• Organism: Klebsiella pneumoniae strain KP36M

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: BK002_RS27670
• Protein ID: WP_077269838.1
• Coordinates: 104507..104989 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: BK002_RS27665
• Protein ID: WP_003026799.1
• Coordinates: 104253..104519 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK002_RS27635	99423..100001	-	579	Protein_116	pcar	-
BK002_RS27640	100349..100612	+	264	WP_009310051.1	hypothetical protein	-
BK002_RS27645	100609..101175	+	567	WP_009654312.1	hypothetical protein	-
BK002_RS27650	101206..101700	+	495	WP_004213594.1	hypothetical protein	-
BK002_RS27655	101761..101964	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
BK002_RS27660	102674..103591	-	918	WP_069985721.1	bifunctional helix-turn-helix transcriptional regulator/GNAT family N-acetyltransferase	-
BK002_RS27665	104253..104519	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
BK002_RS27670	104507..104989	+	483	WP_077269838.1	GNAT family N-acetyltransferase	Toxin
BK002_RS27675	105189..106592	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
BK002_RS27680	106621..107253	-	633	WP_001567369.1	hypothetical protein	-
BK002_RS27685	107483..108829	+	1347	WP_020314316.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(D) / qnrS1 / ant(3'')-Ia / blaTEM-1A	-	1..173923	173923
	-	flank	IS/Tn	-	-	98373..99353	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17378.94 Da
• Isoelectric Point: 9.6047

>T68152 WP_077269838.1 NZ_CP017779:104507-104989 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQRAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T68152 NZ_CP017779:104507-104989 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCGGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT68152 WP_003026799.1 NZ_CP017779:104253-104519 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT68152 NZ_CP017779:104253-104519 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATTGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66