Detailed information of TA system TA068151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 94309..94952
• Replicon: plasmid unnamed2
• Accession: NZ_CP017779
• Organism: Klebsiella pneumoniae strain KP36M

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BK002_RS27620
• Protein ID: WP_069985717.1
• Coordinates: 94536..94952 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: BK002_RS27615
• Protein ID: WP_021312476.1
• Coordinates: 94309..94539 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK002_RS27580	89446..90462	-	1017	WP_017899884.1	hypothetical protein	-
BK002_RS27585	90473..90787	-	315	WP_053389906.1	hypothetical protein	-
BK002_RS27590	90814..91209	-	396	WP_017899885.1	hypothetical protein	-
BK002_RS27595	91378..91683	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
BK002_RS27600	91685..91903	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
BK002_RS27605	92458..93147	+	690	WP_004193995.1	hypothetical protein	-
BK002_RS27610	93179..93868	-	690	WP_069985716.1	RES family NAD+ phosphorylase	-
BK002_RS27615	94309..94539	+	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BK002_RS27620	94536..94952	+	417	WP_069985717.1	type II toxin-antitoxin system VapC family toxin	Toxin
BK002_RS27625	94998..98315	-	3318	WP_069985718.1	pcar	-
BK002_RS27630	98373..99353	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(D) / qnrS1 / ant(3'')-Ia / blaTEM-1A	-	1..173923	173923
	-	flank	IS/Tn	-	-	98373..99353	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14989.47 Da
• Isoelectric Point: 9.2738

>T68151 WP_069985717.1 NZ_CP017779:94536-94952 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T68151 NZ_CP017779:94536-94952 [Klebsiella pneumoniae]
GTGAAAAAGACTTTTATGCTCGACACAAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCCCCGCGCCATATCGAGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCCTTGCGACAGGCCGGGACGCCGATTGGCCCGAACGACACGGCGATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTTCTGGTGACGAACAATGTAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT68151 WP_021312476.1 NZ_CP017779:94309-94539 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT68151 NZ_CP017779:94309-94539 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAATGGCAATAACCGTGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGACGTTGTCAGCGACGAAGGACGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B7Q5S4