Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 91378..91903 Replicon plasmid unnamed2
Accession NZ_CP017779
Organism Klebsiella pneumoniae strain KP36M

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag BK002_RS27595 Protein ID WP_013023785.1
Coordinates 91378..91683 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag BK002_RS27600 Protein ID WP_001568025.1
Coordinates 91685..91903 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK002_RS27565 87073..87699 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
BK002_RS27570 87819..87998 + 180 WP_006788217.1 hypothetical protein -
BK002_RS27575 88454..89248 - 795 WP_004197635.1 site-specific integrase -
BK002_RS27580 89446..90462 - 1017 WP_017899884.1 hypothetical protein -
BK002_RS27585 90473..90787 - 315 WP_053389906.1 hypothetical protein -
BK002_RS27590 90814..91209 - 396 WP_017899885.1 hypothetical protein -
BK002_RS27595 91378..91683 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
BK002_RS27600 91685..91903 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BK002_RS27605 92458..93147 + 690 WP_004193995.1 hypothetical protein -
BK002_RS27610 93179..93868 - 690 WP_069985716.1 RES family NAD+ phosphorylase -
BK002_RS27615 94309..94539 + 231 WP_021312476.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BK002_RS27620 94536..94952 + 417 WP_069985717.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / tet(D) / qnrS1 / ant(3'')-Ia / blaTEM-1A - 1..173923 173923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T68150 WP_013023785.1 NZ_CP017779:c91683-91378 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T68150 NZ_CP017779:c91683-91378 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT68150 WP_001568025.1 NZ_CP017779:c91903-91685 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT68150 NZ_CP017779:c91903-91685 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References