Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 42967..43592 Replicon chromosome
Accession NZ_CP017777
Organism Klebsiella pneumoniae strain KP36M

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag BK002_RS00200 Protein ID WP_002882817.1
Coordinates 43209..43592 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag BK002_RS00195 Protein ID WP_004150355.1
Coordinates 42967..43209 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK002_RS00170 38958..39557 + 600 WP_004151865.1 glucose-1-phosphatase -
BK002_RS00175 39551..40411 + 861 WP_004146232.1 virulence factor BrkB family protein -
BK002_RS00180 40408..40845 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
BK002_RS00185 40890..41831 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
BK002_RS00190 41845..42762 - 918 WP_004181612.1 alpha/beta hydrolase -
BK002_RS00195 42967..43209 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
BK002_RS00200 43209..43592 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
BK002_RS00205 43766..44695 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
BK002_RS00210 44692..45327 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
BK002_RS00215 45324..46226 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T68131 WP_002882817.1 NZ_CP017777:43209-43592 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T68131 NZ_CP017777:43209-43592 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT68131 WP_004150355.1 NZ_CP017777:42967-43209 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT68131 NZ_CP017777:42967-43209 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References