Detailed information of TA system TA068130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1381166..1382083
• Replicon: chromosome
• Accession: NZ_CP017775
• Organism: Bacillus velezensis strain 9912D

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BK055_RS07245
• Protein ID: WP_007407256.1
• Coordinates: 1381337..1382083 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BK055_RS07240
• Protein ID: WP_003154807.1
• Coordinates: 1381166..1381336 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK055_RS07195	1376372..1376884	+	513	WP_007407279.1	sigma-70 family RNA polymerase sigma factor	-
BK055_RS07200	1376997..1377656	+	660	WP_071181722.1	terminase	-
BK055_RS07205	1377554..1378021	+	468	Protein_1371	hypothetical protein	-
BK055_RS07210	1378034..1378405	+	372	WP_038457736.1	YomQ/XkdW protein, phage-like element PBSX	-
BK055_RS07215	1378410..1378607	+	198	WP_007610833.1	XkdX family protein	-
BK055_RS07220	1378664..1379425	+	762	WP_063636818.1	hypothetical protein	-
BK055_RS07225	1379477..1379740	+	264	WP_021493821.1	hemolysin XhlA family protein	-
BK055_RS07230	1379754..1380017	+	264	WP_003154813.1	phage holin	-
BK055_RS07235	1380031..1380909	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BK055_RS07240	1381166..1381336	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BK055_RS07245	1381337..1382083	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BK055_RS07250	1382187..1383185	-	999	WP_017417610.1	inorganic phosphate transporter	-
BK055_RS07255	1383198..1383815	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BK055_RS07260	1384102..1385418	-	1317	WP_063636817.1	amino acid permease	-
BK055_RS07265	1385740..1386690	+	951	WP_071181723.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1355925..1440379	84454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T68130 WP_007407256.1 NZ_CP017775:c1382083-1381337 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T68130 NZ_CP017775:c1382083-1381337 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT68130 WP_003154807.1 NZ_CP017775:c1381336-1381166 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT68130 NZ_CP017775:c1381336-1381166 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14