Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 482304..482941 Replicon chromosome
Accession NZ_CP017775
Organism Bacillus velezensis strain 9912D

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BK055_RS02445 Protein ID WP_003156187.1
Coordinates 482591..482941 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BK055_RS02440 Protein ID WP_003156188.1
Coordinates 482304..482585 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BK055_RS02420 478669..479268 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
BK055_RS02425 479361..479726 + 366 WP_007609580.1 holo-ACP synthase -
BK055_RS02430 479891..480898 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BK055_RS02435 481015..482184 + 1170 WP_065180972.1 alanine racemase -
BK055_RS02440 482304..482585 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BK055_RS02445 482591..482941 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BK055_RS02450 483059..483880 + 822 WP_003156182.1 STAS domain-containing protein -
BK055_RS02455 483885..484250 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
BK055_RS02460 484253..484654 + 402 WP_003156178.1 anti-sigma regulatory factor -
BK055_RS02465 484666..485673 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
BK055_RS02470 485737..486066 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BK055_RS02475 486063..486545 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BK055_RS02480 486511..487299 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BK055_RS02485 487299..487901 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T68129 WP_003156187.1 NZ_CP017775:482591-482941 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T68129 NZ_CP017775:482591-482941 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT68129 WP_003156188.1 NZ_CP017775:482304-482585 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT68129 NZ_CP017775:482304-482585 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References