Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1775273..1775909 Replicon chromosome
Accession NZ_CP017763
Organism Bacillus subtilis strain 29R7-12

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BKP58_RS09695 Protein ID WP_003156187.1
Coordinates 1775273..1775623 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BKP58_RS09700 Protein ID WP_003225183.1
Coordinates 1775628..1775909 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKP58_RS09655 1770309..1770908 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
BKP58_RS09660 1770908..1771696 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
BKP58_RS09665 1771662..1772144 - 483 WP_003234299.1 anti-sigma B factor RsbW -
BKP58_RS09670 1772141..1772470 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
BKP58_RS09675 1772539..1773546 - 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
BKP58_RS09680 1773558..1773959 - 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
BKP58_RS09685 1773963..1774328 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
BKP58_RS09690 1774333..1775157 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BKP58_RS09695 1775273..1775623 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BKP58_RS09700 1775628..1775909 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BKP58_RS09705 1776025..1777194 - 1170 WP_014478898.1 alanine racemase -
BKP58_RS09710 1777309..1778325 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
BKP58_RS09715 1778491..1778856 - 366 WP_014478897.1 holo-ACP synthase -
BKP58_RS09720 1778951..1779550 + 600 WP_014478896.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T68127 WP_003156187.1 NZ_CP017763:c1775623-1775273 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T68127 NZ_CP017763:c1775623-1775273 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT68127 WP_003225183.1 NZ_CP017763:c1775909-1775628 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT68127 NZ_CP017763:c1775909-1775628 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References