Detailed information of TA system TA068123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 1624265..1624913
• Replicon: chromosome
• Accession: NZ_CP017762
• Organism: Virgibacillus sp. 6R

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0L0QSJ8
• Locus tag: BKP57_RS07440
• Protein ID: WP_050351134.1
• Coordinates: 1624265..1624624 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BKP57_RS07445
• Protein ID: WP_077298901.1
• Coordinates: 1624629..1624913 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKP57_RS07410	1619502..1619837	-	336	WP_050351140.1	STAS domain-containing protein	-
BKP57_RS07415	1620189..1621202	-	1014	WP_050351139.1	PP2C family protein-serine/threonine phosphatase	-
BKP57_RS07420	1621384..1621785	-	402	WP_050351138.1	anti-sigma regulatory factor	-
BKP57_RS07425	1621789..1622154	-	366	WP_170875213.1	STAS domain-containing protein	-
BKP57_RS07430	1622151..1623020	-	870	WP_072741261.1	RsbT co-antagonist protein RsbRA	-
BKP57_RS07440	1624265..1624624	-	360	WP_050351134.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BKP57_RS07445	1624629..1624913	-	285	WP_077298901.1	antitoxin	Antitoxin
BKP57_RS07450	1625312..1626448	-	1137	WP_072742392.1	alanine racemase	-
BKP57_RS07455	1627023..1628045	-	1023	WP_072741263.1	outer membrane lipoprotein carrier protein LolA	-
BKP57_RS07460	1628211..1629731	-	1521	WP_072741264.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13455.69 Da
• Isoelectric Point: 8.4535

>T68123 WP_050351134.1 NZ_CP017762:c1624624-1624265 [Virgibacillus sp. 6R]
MIVQRGEVYFADLSPVVGSEQGGIRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFDRDSVILLEQI
RTLDKQRLTDKITKLDKEMMKKINQALEISLGLKDMYDQ

Nucleotide

• Length: 360 bp

>T68123 NZ_CP017762:c1624624-1624265 [Virgibacillus sp. 6R]
TTGATCGTACAAAGAGGCGAAGTGTATTTCGCTGACCTTTCCCCTGTTGTTGGATCAGAACAGGGAGGCATCCGCCCGGT
ATTGATCCTGCAAAATGATATCGGGAATCGTTTCAGTCCTACCGTTATTGTTGCTGCAATTACAGCGCAAATTCAAAAAG
CAAAGCTCCCTACTCATGTGGAGATTGATGCAAAACGATATGGGTTTGACCGTGATTCCGTTATTTTGCTAGAGCAAATA
CGAACACTTGATAAACAACGGTTAACAGATAAAATTACAAAATTAGATAAAGAAATGATGAAAAAGATCAACCAGGCATT
GGAAATCAGTTTAGGACTGAAGGATATGTATGATCAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10867.21 Da
• Isoelectric Point: 4.5792

>AT68123 WP_077298901.1 NZ_CP017762:c1624913-1624629 [Virgibacillus sp. 6R]
MSESLQEVLVKIPKNLLNEVDGLVKYENSDLSDFICQATRNYLQHKRDEHFQQFRETMEKGYAEMGRINLTIASEAFQAE
EEAKNTLERSVIGV

Nucleotide

• Length: 285 bp

>AT68123 NZ_CP017762:c1624913-1624629 [Virgibacillus sp. 6R]
TTGTCAGAAAGCTTGCAAGAAGTATTAGTTAAAATACCGAAAAACCTGTTAAATGAGGTGGATGGTCTGGTGAAGTATGA
GAATAGTGATTTGAGTGATTTCATATGTCAGGCAACAAGGAACTATCTTCAGCATAAAAGAGATGAGCACTTTCAACAAT
TTCGCGAAACCATGGAAAAAGGTTATGCAGAAATGGGTCGTATTAATTTGACAATTGCTTCAGAAGCTTTTCAAGCTGAA
GAGGAAGCAAAAAACACCTTGGAACGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0L0QSJ8