Detailed information of TA system TA068112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3889..4405
• Replicon: plasmid unnamed1
• Accession: NZ_CP017756
• Organism: Cupriavidus malaysiensis strain USMAA1020 isolate pure

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BKK80_RS34500
• Protein ID: WP_071073587.1
• Coordinates: 3889..4173 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BKK80_RS34505
• Protein ID: WP_071073885.1
• Coordinates: 4163..4405 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK80_RS34490	440..1222	-	783	WP_157903441.1	hypothetical protein	-
BKK80_RS36040	1312..1647	-	336	WP_157903442.1	TonB C-terminal domain-containing protein	-
BKK80_RS36045	2191..3669	-	1479	WP_157903443.1	RES family NAD+ phosphorylase	-
BKK80_RS34500	3889..4173	-	285	WP_071073587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKK80_RS34505	4163..4405	-	243	WP_071073885.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BKK80_RS34510	4655..5813	-	1159	Protein_6	transposase	-
BKK80_RS36895	5968..6279	-	312	WP_157903444.1	hypothetical protein	-
BKK80_RS34515	6635..6922	-	288	WP_071073591.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..176879	176879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10786.62 Da
• Isoelectric Point: 10.1294

>T68112 WP_071073587.1 NZ_CP017756:c4173-3889 [Cupriavidus malaysiensis]
MSYSLQFNVAALKEWGKLDRPIREQFKKKLAERLEHPHVAASRLHGMTDCYKIKLQAAGYRLVYQVHDEHVVVVVIAVGK
RDAGRVYAIALDRL

Nucleotide

• Length: 285 bp

>T68112 NZ_CP017756:c4173-3889 [Cupriavidus malaysiensis]
ATGAGCTATAGCCTCCAATTCAATGTTGCCGCCCTCAAAGAATGGGGCAAGCTGGATCGGCCGATCCGCGAACAGTTCAA
GAAAAAGCTTGCCGAGCGCCTGGAGCACCCGCACGTAGCCGCCTCGCGGCTGCATGGCATGACCGACTGCTACAAGATCA
AGCTGCAAGCGGCTGGCTACCGGTTGGTCTACCAGGTTCATGATGAGCACGTTGTGGTCGTCGTGATCGCGGTCGGCAAG
CGCGACGCGGGGCGCGTCTATGCGATCGCGCTGGATCGTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8744.02 Da
• Isoelectric Point: 4.1948

>AT68112 WP_071073885.1 NZ_CP017756:c4405-4163 [Cupriavidus malaysiensis]
METILARASIGISELKVNPTATIEQADGPVAVLNRNKPVAYLIPASDWEAIHELLEDIELARIVREREGAPTVPVTLDEL

Nucleotide

• Length: 243 bp

>AT68112 NZ_CP017756:c4405-4163 [Cupriavidus malaysiensis]
ATGGAAACCATACTTGCCCGCGCCTCTATCGGCATCAGTGAACTCAAGGTGAACCCAACGGCCACCATCGAGCAAGCGGA
TGGTCCGGTGGCGGTACTCAACCGCAACAAGCCTGTCGCCTACCTGATTCCGGCCTCCGACTGGGAGGCCATCCACGAGC
TGCTGGAGGATATCGAGCTCGCGCGGATCGTCAGAGAGCGCGAAGGGGCGCCTACCGTACCCGTCACGCTCGATGAGCTA
TAG